Exercise-acclimated microbiota improves skeletal muscle metabolism via circulating bile acid deconjugation

• Published in: iScience 2023-03, Vol.26 (3), p.106251-106251, Article 106251
• Main Authors: Aoi, Wataru, Inoue, Ryo, Mizushima, Katsura, Honda, Akira, Björnholm, Marie, Takagi, Tomohisa, Naito, Yuji
• Format: Article
• Language: English
• Subjects: Medicin och hälsovetenskap; Microbial metabolism; Microbiome; Musculoskeletal medicine
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2023.106251

Habitual exercise alters the intestinal microbiota composition, which may mediate its systemic benefits. We examined whether transplanting fecal microbiota from trained mice improved skeletal muscle metabolism in high-fat diet (HFD)-fed mice. Fecal samples from sedentary and exercise-trained mice were gavage-fed to germ-free mice. After receiving fecal samples from trained donor mice for 1 week, recipient mice had elevated levels of AMP-activated protein kinase (AMPK) and insulin growth factor-1 in skeletal muscle. In plasma, bile acid (BA) deconjugation was found to be promoted in recipients transplanted with feces from trained donor mice; free-form BAs also induced more AMPK signaling and glucose uptake than tauro-conjugated BAs. The transplantation of exercise-acclimated fecal microbiota improved glucose tolerance after 8 weeks of HFD administration. Intestinal microbiota may mediate exercise-induced metabolic improvements in mice by modifying circulating BAs. Our findings provide insights into the prevention and treatment of metabolic diseases. [Display omitted] •Habitual exercise modulates the composition of the gut microbiota•The modulation of microbiota ameliorates diet-induced metabolic disturbance•Deconjugated BAs activate AMPK signaling and glucose uptake in skeletal muscle•Altered microbiota improves metabolism by modifying circulating bile acids in mice Musculoskeletal medicine; Microbiome; Microbial metabolism