IL-17 Genetic and Immunophenotypic Evaluation in Chronic Graft-versus-Host Disease

Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes po...

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Bibliographic Details
Published in:Mediators of Inflammation 2014-01, Vol.2014 (5), p.468-474
Main Authors: Gomez, Ricardo Santiago, Vieira, Érica Leandro Marciano, Marques-Silva, Luciano, Salomão, Ulisses Eliezer, Silva, Tarcília Aparecida da, Correia-Silva, Jeane de Fátima, Resende, Renata Gonçalves, Dutra, Walderez Ornelas
Format: Article
Language:English
Subjects:
Adolescent
Adult
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Gene expression
Genotype
Graft versus host reaction
Graft vs Host Disease - genetics
Health aspects
Humans
Interleukin-17 - genetics
Interleukins
Male
Middle Aged
Phenotype
Polymorphism, Genetic - genetics
Young Adult
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Summary:Although interleukin-17 (IL-17) is a recently discovered cytokine associated with several autoimmune diseases, its role in the pathogenesis of chronic graft-versus-host disease (cGVHD) was not established yet. The objective of this study was to investigate the association of IL17A and IL17F genes polymorphisms and IL-17A and IL-17F levels with cGVHD. IL-17A expression was also investigated in CD4+ T cells of patients with systemic cGVHD. For Part I of the study, fifty-eight allo-HSCT recipients and donors were prospectively studied. Blood samples were obtained to determine IL17A and IL17F genes polymorphisms. Cytokines levels in blood and saliva were assessed by ELISA at days +35 and +100 after HSCT. In Part II, for the immunophenotypic evaluation, eight patients with systemic cGVHD were selected and the expression of IL-17A was evaluated. We found association between recipient AA genotype with systemic cGVHD. No association was observed between IL-17A levels and cGVHD. Lower IL-17A levels in the blood were associated with AA genotype. In flow cytometry analysis, decreased expression of IL-17A was observed in patients with cGVHD after stimulation. In conclusion, IL-17A may have an important role in the development of systemic cGVHD.
ISSN:0962-9351
1466-1861
DOI:10.1155/2014/571231