Multiple F-Box Proteins Collectively Regulate Cell Development and Pathogenesis in the Human Pathogen Cryptococcus neoformans

The ubiquitin-proteasome system (UPS) mediates intracellular proteins degradation that influences various cellular functions in eukaryotic cells. The UPS is also involved in the development and virulence of pathogenic fungi. F-box proteins, which are part of the SCF (Skp1-Cullin-F-box protein) ligas...

Full description

Saved in:
Bibliographic Details
Published in:Journal of fungi (Basel) 2022-11, Vol.8 (12), p.1259
Main Authors: Cao, Chengjun, Wang, Yina, Avina, Samantha L, Walter, John, Xue, Chaoyang
Format: Article
Language:English
Subjects:
Adaptation
Cell size
Cryptococcus neoformans
Cullin
Deletion mutant
Drug resistance
E3 ligase
Enzymes
F-box protein
Fungal infections
fungal pathogenesis
Genes
Genomes
Genotype & phenotype
Meningitis
Mutants
Pathogenesis
Pathogens
Proteasomes
Proteins
Sexual reproduction
stress response
Ubiquitin
Ubiquitin-protein ligase
Virulence
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ubiquitin-proteasome system (UPS) mediates intracellular proteins degradation that influences various cellular functions in eukaryotic cells. The UPS is also involved in the development and virulence of pathogenic fungi. F-box proteins, which are part of the SCF (Skp1-Cullin-F-box protein) ligase, are a key component of UPS and are essential for the recognition of specific substrates. In this study, we identified 20 F-box proteins in and obtained deletion mutants for 19 of them. A comprehensive phenotypic analysis of these mutants revealed the diverse function of F-box proteins in stress response, cell size regulation, sexual reproduction, antifungal drug resistance, and fungal virulence in . The importance of three F-box proteins: Fbp4, Fbp8, and Fbp11, in these cellular functions were characterized in detail. This study provides an overall view of the F-box gene family in , which will lead to a better understanding of the function of fungal SCF E3 ligase-mediated UPS in fungal development and pathogenesis.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof8121259