A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage

• Published in: Nature communications 2017-09, Vol.8 (1), p.676-9, Article 676
• Main Authors: Shan, Chao, Muruato, Antonio E., Jagger, Brett W., Richner, Justin, Nunes, Bruno T. D., Medeiros, Daniele B. A., Xie, Xuping, Nunes, Jannyce G. C., Morabito, Kaitlyn M., Kong, Wing-Pui, Pierson, Theodore C., Barrett, Alan D., Weaver, Scott C., Rossi, Shannan L., Vasconcelos, Pedro F. C., Graham, Barney S., Diamond, Michael S., Shi, Pei-Yong
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; 631/326/590/1867; 631/326/596/2555; 692/699/255/2514; Adults; Animal models; Animals; Antibody response; Attenuation; Damage prevention; Disease transmission; Drug Evaluation, Preclinical; Embryos; Female; Fetuses; Flavivirus; Genomes; Humanities and Social Sciences; Immunization; Infections; Infectious Disease Transmission, Vertical - prevention & control; Male; Mice; Mice, Inbred C57BL; multidisciplinary; Placenta; Pregnancy; Primates; Reproductive system; Ribonucleic acid; RNA; Rodents; Science; Science (multidisciplinary); Sexual transmission; Testis - pathology; Testis - virology; Vaccination; Vaccines; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - adverse effects; Vaccines, Attenuated - therapeutic use; Vector-borne diseases; Viral Vaccines - administration & dosage; Viral Vaccines - adverse effects; Viral Vaccines - therapeutic use; Viruses; Zika virus; Zika Virus - genetics; Zika Virus - immunology; Zika Virus Infection - prevention & control; Zika Virus Infection - transmission
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-017-00737-8

Zika virus infection during pregnancy can cause congenital abnormities or fetal demise. The persistence of Zika virus in the male reproductive system poses a risk of sexual transmission. Here we demonstrate that live-attenuated Zika virus vaccine candidates containing deletions in the 3′ untranslated region of the Zika virus genome (ZIKV-3′UTR-LAV) prevent viral transmission during pregnancy and testis damage in mice, as well as infection of nonhuman primates. After a single-dose vaccination, pregnant mice challenged with Zika virus at embryonic day 6 and evaluated at embryonic day 13 show markedly diminished levels of viral RNA in maternal, placental, and fetal tissues. Vaccinated male mice challenged with Zika virus were protected against testis infection, injury, and oligospermia. A single immunization of rhesus macaques elicited a rapid and robust antibody response, conferring complete protection upon challenge. Furthermore, the ZIKV-3′UTR-LAV vaccine candidates have a desirable safety profile. These results suggest that further development of ZIKV-3′UTR-LAV is warranted for humans. Zika virus infection can result in congenital disorders and cause disease in adults, and there is currently no approved vaccine. Here Shan et al. show that a single dose of a live-attenuated Zika vaccine prevents infection, testis damage and transmission to the fetus during pregnancy in different animal models.