Prevalence of aflatoxin, ochratoxin and deoxynivalenol in cereal grains in northern Uganda: Implication for food safety and health

• Published in: Toxicology reports 2019-01, Vol.6, p.1012-1017
• Main Authors: Echodu, Richard, Maxwell Malinga, Geoffrey, Moriku Kaducu, Joyce, Ovuga, Emilio, Haesaert, Geert
• Format: Article
• Language: English
• Subjects: Aflatoxin; aflatoxins; average daily intake; corn; Deoxynivalenol; enzyme-linked immunosorbent assay; European Union; Food grains; Food safety; millets; Ochratoxin; ochratoxins; risk; toxicology; Uganda
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2019.09.002

[Display omitted] •We assessed the levels of aflatoxin, total ochratoxin and deoxynivalenol contamination in 105 samples of food grains in northern Uganda.•The total aflatoxin limits in the foods analysed varied from not detected to 68.2 μg/Kg while ochratoxin levels ranged between 0.1 to 16.4 μg/Kg, whereas DON was from not detected to 2606 μg/Kg.•46.5% of the sorghum consumed in both districts exceeded the maximum tolerable limit of 10 μg/Kg for Uganda national regulatory limit.•86.1% of the sorghum consumed in both districts exceeded European Union maximum tolerable limit of 4 μg/Kg.•In conclusion, consumption of cereals cultivated in this region poses no health risk of mycotoxins exposure since HI values obtained were less than 1. Mycotoxin contamination of cereals is a significant health risk for humans and animals, particularly in developing countries. To gain insight into food safety related to agricultural practices, we assessed levels of mycotoxin contamination in 105 samples of food grains raised and stored for consumption by rural households in the post-conflict districts of Kitgum and Lamwo in Northern Uganda. Aflatoxin, ochratoxin and deoxynivalenol (DON) contamination was assessed by quantitative enzyme-linked immunosorbent assay. Total aflatoxin in the foods analyzed varied from nd (not detected) to 68.2 μg/Kg. Ochratoxin ranged from 0.1 to 16.4 μg/Kg. DON ranged from nd to 2606 μg/Kg. The mean concentration of total aflatoxins was significantly higher (P = 0.002) in sorghum than in millet, maize and sesame seeds. Frequency of co-occurrence of two mycotoxins ranged from 8.3 to 100%, with the highest being aflatoxin and ochratoxin in sorghum. Co-occurrence of all three mycotoxins ranged from 8.3 to 35.3%, with the highest again being in sorghum. Mean levels of aflatoxins concentration in sorghum samples were 11.8 μg/Kg, exceeding the Ugandan national regulatory limits of 10 μg/Kg. Furthermore, 46.5% of the sorghum consumed in both districts exceeded this limit, and 86.1% of sorghum samples exceeded the European Union (E.U.) maximum tolerable limit of 4 μg/Kg. The Estimated Daily Intake (EDI) and Hazard Indices (HI) values were in the range of 1.2 × 10−5–91.521 and 1.3 × 10−7 to 0.0059, respectively. In conclusion, our results provide evidence of high levels of mycotoxin contamination and co-occurrence in food grains in Northern Uganda with aflatoxins and ochratoxins at high levels in all the cereal types analyzed. Consumption of cereals culti