Methylation-specific PCR unraveled

Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whe...

Full description

Saved in:
Bibliographic Details
Published in:Analytical cellular pathology (Amsterdam) 2004, Vol.26 (5-6), p.291-299
Main Authors: Derks, Sarah, Lentjes, Marjolein H F M, Hellebrekers, Debby M E I, de Bruïne, Adriaan P, Herman, James G, van Engeland, Manon
Format: Article
Language:English
Subjects:
Biomarkers, Tumor
Chromatography, High Pressure Liquid
CpG Islands
Cytosine - chemistry
DNA Methylation
DNA Primers - chemistry
Gene Silencing
Humans
Other
Polymerase Chain Reaction - methods
Software
Sulfites - pharmacology
Uracil - chemistry
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.
ISSN:1570-5870
2210-7177
2210-7185
1875-8606
DOI:10.1155/2004/370301