Cytokine biomarkers in tear film for primary open-angle glaucoma

• Published in: Clinical ophthalmology (Auckland, N.Z.) N.Z.), 2017-01, Vol.11, p.411-416
• Main Authors: Gupta, Divakar, Wen, Joanne C, Huebner, Janet L, Stinnett, Sandra, Kraus, Virginia B, Tseng, Henry C, Walsh, Molly
• Format: Article
• Language: English
• Subjects: Automation; Biomarkers; Care and treatment; Cataracts; Cytokines; Development and progression; Genetic aspects; Glaucoma; glaucoma diagnosis; Growth factors; Health aspects; Measurement techniques; Open-angle glaucoma; Original Research; Oxidative stress; Proteins; tear film; Tumor necrosis factor-TNF
• ISSN: 1177-5467; 1177-5483; 1177-5483
• DOI: 10.2147/OPTH.S125364

To determine the utility of tear film cytokines as biomarkers for early primary open-angle glaucoma (POAG). Patients without POAG and eye drop-naïve patients with newly diagnosed POAG were recruited from an academic hospital-based glaucoma practice. Tear films of recruited patients were obtained and analyzed using a multiplex, high-sensitivity electrochemiluminescent enzyme-linked immunosorbent assay for proinflammatory cytokines (IFNγ, IL-10, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-6, IL-8, and TNFα). Mean concentrations of tear film cytokines were lower in the glaucoma group for 8 of 10 cytokines tested. IL-12p70 (3.94±2.19 pg/mL in control vs 2.31±1.156 pg/mL in POAG; =0.035) was significantly lower in the tear film of patients with newly diagnosed POAG. Proinflammatory cytokines were lower in eye drop-naïve newly diagnosed glaucoma patients. Tear film cytokine profiles may be used as biomarkers of early POAG.