The Spread of NDM-1 and NDM-7-Producing Klebsiella pneumoniae Is Driven by Multiclonal Expansion of High-Risk Clones in Healthcare Institutions in the State of Pará, Brazilian Amazon Region

• Published in: Antibiotics (Basel) 2021-12, Vol.10 (12), p.1527
• Main Authors: Rodrigues, Yan Corrêa, Lobato, Amália Raiana Fonseca, Quaresma, Ana Judith Pires Garcia, Guerra, Lívia Maria Guimarães Dutra, Brasiliense, Danielle Murici
• Format: Article
• Language: English
• Subjects: Antimicrobial agents; Antimicrobial resistance; Carbapenemase; Carbapenems; Cephalosporins; Cloning; Coronaviruses; COVID-19; Disease transmission; Drug resistance; E coli; Epidemics; Epidemiology; Fluoroquinolones; Genes; Genotyping; Gram-negative bacteria; Health care; Impact resistance; Intensive care; Klebsiella; Klebsiella pneumoniae; Metallo-β-lactamase; Metallography; molecular epidemiology; multi-drug resistance; Multidrug resistance; Pandemics; Pediatrics; Surveillance; β Lactamase
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics10121527

Carbapenem resistance among isolates is often related to carbapenemase genes, located in genetic transmissible elements, particularly the gene, which variants are spread in several countries. Recently, reports of isolates harboring the gene have increased dramatically along with the dissemination of epidemic high-risk clones (HRCs). In the present study, we report the multiclonal spread of New Delhi metallo-beta-lactamase (NDM)-producing in different healthcare institutions in the state of Pará, Northern Brazil. A total of 23 NDM-producing isolates were tested regarding antimicrobial susceptibility testing features, screening of carbapenemase genes, and genotyping by multilocus sequencing typing (MLST). All isolates were determined as multidrug-resistant (MDR), being mainly resistant to carbapenems, cephalosporins, and fluoroquinolones. The (60.9%-14/23) and (34.8%-8/23) variants were detected. MLST genotyping revealed the predomination of HRCs, including ST11/CC258, ST340/CC258, ST15/CC15, ST392/CC147, among others. To conclude, the present study reveals the contribution of HRCs and non-HRCs in the spread of NDM-1 and NDM-7-producing isolates in Northern (Amazon region) Brazil, along with the first detection of NDM-7 variant in Latin America and Brazil, highlighting the need for surveillance and control of strains that may negatively impact healthcare and antimicrobial resistance.