Genome-Wide Identification and Characterization of the Shaker-Type K+ Channel Genes in Prunus persica (L.) Batsch

• Published in: International journal of genomics 2022-03, Vol.2022, p.5053838-11
• Main Authors: Yang, Yong, Han, Jinlong, Zhang, Yue, Lin, Shizhuo, Liang, Meixia, Zhao, Lizi, Song, Zhizhong
• Format: Article
• Language: English
• Subjects: Abscisic acid; Flowers; Flowers & plants; Fruit trees; Fruits; Gene expression; Genomes; Independent sample; Ions; Molecular modelling; Phylogenetics; Phylogeny; Potassium channels (voltage-gated); Proteins; Regulatory sequences; Seedlings; Sodium chloride; Sorghum; Statistical analysis; Trees
• ISSN: 2314-436X; 2314-4378
• DOI: 10.1155/2022/5053838

Shaker-type K+ channels are critical for plant K+ acquisition and translocation that play key roles during plant growth and development. However, molecular mechanisms towards K+ channels are extremely rare in fruit trees, especially in peach. In this study, we identified 7 putative shaker-type K+ channel genes from peach, which were unevenly distributed on 5 chromosomes. The peach shaker K+ channel proteins were classified into 5 subfamilies, I-V, and were tightly clustered with pear homologs in the phylogenetic tree. Various cis-acting regulatory elements were detected in the promoter region of the shaker-type K+ channel genes, including phytohormone-responsive, abiotic stress-responsive, and development regulatory elements. The peach shaker K+ channel genes were expressed differentially in distinct tissues, and PpSPIK was specifically expressed in the full-bloom flowers; PpKAT1 and PpGORK were predominantly expressed in the leaves, while PpAKT1, PpKC1, and PpSKOR were majorly expressed in the roots. The peach shaker K+ channel genes were differentially regulated by abiotic stresses in that K+ deficiency, and ABA treatment mainly increased the shaker K+ channel gene expression throughout the whole seedling, whereas NaCl and PEG treatment reduced the shaker K+ channel gene expression, especially in the roots. Moreover, electrophysiological analysis demonstrated that PpSKOR is a typical voltage-dependent outwardly rectifying K+ channel in peach. This study lays a molecular basis for further functional studies of the shaker-type K+ channel genes in peach and provides a theoretical foundation for K+ nutrition and balance research in fruit trees.