Microfluidic Synthesis of Magnetite Nanoparticles for the Controlled Release of Antibiotics

• Published in: Pharmaceutics 2023-08, Vol.15 (9), p.2215
• Main Authors: Chircov, Cristina, Dumitru, Iulia Alexandra, Vasile, Bogdan Stefan, Oprea, Ovidiu-Cristian, Holban, Alina Maria, Popescu, Roxana Cristina
• Format: Article
• Language: English
• Subjects: Analysis; Antibiotics; Antimicrobial agents; Cell death; Drug delivery systems; Drugs; Fibroblasts; Health aspects; Investigations; Magnetite; magnetite nanoparticles; Mercury cadmium telluride detectors; microfluidics; Nanoparticles; Neomycin; Nosocomial infections; Particle size; Radiation; Software; Spectrum analysis; Vehicles
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics15092215

Magnetite nanoparticles (MNPs) have been intensively studied for biomedical applications, especially as drug delivery systems for the treatment of infections. Additionally, they are characterized by intrinsic antimicrobial properties owing to their capacity to disrupt or penetrate the microbial cell wall and induce cell death. However, the current focus has shifted towards increasing the control of the synthesis reaction to ensure more uniform nanoparticle sizes and shapes. In this context, microfluidics has emerged as a potential candidate method for the controlled synthesis of nanoparticles. Thus, the aim of the present study was to obtain a series of antibiotic-loaded MNPs through a microfluidic device. The structural properties of the nanoparticles were investigated through X-ray diffraction (XRD) and, selected area electron diffraction (SAED), the morphology was evaluated through transmission electron microscopy (TEM) and high-resolution TEM (HR-TEM), the antibiotic loading was assessed through Fourier-transform infrared spectroscopy (FT-IR) and, and thermogravimetry and differential scanning calorimetry (TG-DSC) analyses, and. the release profiles of both antibiotics was determined through UV-Vis spectroscopy. The biocompatibility of the nanoparticles was assessed through the MTT assay on a BJ cell line, while the antimicrobial properties were investigated against the S. aureus, P. aeruginosa, and C. albicans strains. Results proved considerable uniformity of the antibiotic-containing nanoparticles, good biocompatibility, and promising antimicrobial activity. Therefore, this study represents a step forward towards the microfluidic development of highly effective nanostructured systems for antimicrobial therapies.