Ethyl pyruvate ameliorate inflammatory response of sinonasal mucosa by inhibiting HMGB1 in rats with acute rhinosinusitis

High mobility group box 1 (HMGB1) has been known to involve in the pathogenesis of many inflammatory diseases. The aim of this study was to establish animal model of acute rhinosinusitis (ARS), and determine whether ethyl pyruvate (EP) attenuate inflammatory response of sinonasal mucosa by inhibitin...

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Bibliographic Details
Published in:Scientific reports 2021-03, Vol.11 (1), p.6206-6206, Article 6206
Main Authors: Liang, Xiang, Shen, Yang, Zhang, Xiaowei, He, Guangxiang, Tan, Guolin
Format: Article
Language:English
Subjects:
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692/4017
Animal models
Bacteria
Enzyme-linked immunosorbent assay
HMGB1 protein
Humanities and Social Sciences
Immunohistochemistry
Inflammation
Inflammatory diseases
Interleukin 6
Ionization
Ions
Mass spectrometry
Mass spectroscopy
Mucosa
multidisciplinary
Polymerase chain reaction
Pyruvic acid
Rhinitis
Rhinosinusitis
Rodents
Science
Science (multidisciplinary)
Serum levels
Sinusitis
Tumor necrosis factor-α
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Summary:High mobility group box 1 (HMGB1) has been known to involve in the pathogenesis of many inflammatory diseases. The aim of this study was to establish animal model of acute rhinosinusitis (ARS), and determine whether ethyl pyruvate (EP) attenuate inflammatory response of sinonasal mucosa by inhibiting HMGB1 in ARS animals. Thirty-six Sprague Dawley (SD) rat were used as follows: six normal controls without intervention (group 1); thirty rats were used for establishment of ARS rats model by nasal insertion of Merocel sponge, and model rats without any treatments (group 2), treated with nasal drops of sterile saline (group 3), 10 μl EP (group 4), and 20 μl EP (group 5), twice a day for 5 days, respectively. Bacterial culture was done regularly and the main bacterial strains were identified using matrix-assisted laser desorption/ionization time of flight mass spectrometry. HMGB1 expression in sinonasal mucosa was detected by immunohistochemistry and RT-PCR. Serum levels of HMGB1, IL-6, and TNF-α were determined by ELISA. Data from 29 of 36 rats that had completed research were analyzed. Bacterial colony formation unit (CFU) of nasal secretion was significantly higher in each group of ARS rats compared with controls (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-85785-3