Voltage-gated Na + currents in human dorsal root ganglion neurons

Available evidence indicates voltage-gated Na channels (VGSCs) in peripheral sensory neurons are essential for the pain and hypersensitivity associated with tissue injury. However, our understanding of the biophysical and pharmacological properties of the channels in sensory neurons is largely based...

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Bibliographic Details
Published in:eLife 2017-05, Vol.6
Main Authors: Zhang, Xiulin, Priest, Birgit T, Belfer, Inna, Gold, Michael S
Format: Article
Language:English
Subjects:
Animals
Cations - metabolism
cell culture
Dorsal root ganglia
Drug development
Ganglia, Spinal - metabolism
Humans
Hypersensitivity
Local anesthesia
local anesthetics
Males
NaV1.7
NaV1.8
Neurons
Neurons - metabolism
Neuroscience
Neurosciences
Pain
Patch-Clamp Techniques
Rats
Rodents
Sensory neurons
Sodium - metabolism
Sodium channels
Sodium channels (voltage-gated)
Tetrodotoxin
Voltage-Gated Sodium Channels - metabolism
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Summary:Available evidence indicates voltage-gated Na channels (VGSCs) in peripheral sensory neurons are essential for the pain and hypersensitivity associated with tissue injury. However, our understanding of the biophysical and pharmacological properties of the channels in sensory neurons is largely based on the study of heterologous systems or rodent tissue, despite evidence that both expression systems and species differences influence these properties. Therefore, we sought to determine the extent to which the biophysical and pharmacological properties of VGSCs were comparable in rat and human sensory neurons. Whole cell patch clamp techniques were used to study Na currents in acutely dissociated neurons from human and rat. Our results indicate that while the two major current types, generally referred to as tetrodotoxin (TTX)-sensitive and TTX-resistant were qualitatively similar in neurons from rats and humans, there were several differences that have important implications for drug development as well as our understanding of pain mechanisms.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.23235