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A bacterial kinase phosphorylates OSK1 to suppress stomatal immunity in rice

The Xanthomonas outer protein C2 (XopC2) family of bacterial effectors is widely found in plant pathogens and Legionella species. However, the biochemical activity and host targets of these effectors remain enigmatic. Here we show that ectopic expression of XopC2 promotes jasmonate signaling and sto...

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Published in:Nature communications 2021-09, Vol.12 (1), p.5479-5479, Article 5479
Main Authors: Wang, Shanzhi, Li, Shuai, Wang, Jiyang, Li, Qian, Xin, Xiu-Fang, Zhou, Shuang, Wang, Yanping, Li, Dayong, Xu, Jiaqing, Luo, Zhao-Qing, He, Sheng Yang, Sun, Wenxian
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Language:English
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Summary:The Xanthomonas outer protein C2 (XopC2) family of bacterial effectors is widely found in plant pathogens and Legionella species. However, the biochemical activity and host targets of these effectors remain enigmatic. Here we show that ectopic expression of XopC2 promotes jasmonate signaling and stomatal opening in transgenic rice plants, which are more susceptible to Xanthomonas oryzae pv. oryzicola infection. Guided by these phenotypes, we discover that XopC2 represents a family of atypical kinases that specifically phosphorylate OSK1, a universal adaptor protein of the Skp1-Cullin-F-box ubiquitin ligase complexes. Intriguingly, OSK1 phosphorylation at Ser 53 by XopC2 exclusively increases the binding affinity of OSK1 to the jasmonate receptor OsCOI1b, and specifically enhances the ubiquitination and degradation of JAZ transcription repressors and plant disease susceptibility through inhibiting stomatal immunity. These results define XopC2 as a prototypic member of a family of pathogenic effector kinases and highlight a smart molecular mechanism to activate jasmonate signaling. XopC2 effectors are present in many plant bacterial pathogens. Here the authors show that XopC2 has kinase activity and enhances disease susceptibility by phosphorylating the OSK1 protein, which increases its interaction with the jasmonate receptor COI1b to promote JA signaling and stomatal opening.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-25748-4