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Temporal and fertilizer-dependent dynamics of soil bacterial communities in buckwheat fields under long-term management
This study integrated bacterial community and soil chemicals to characterize the soil ecosystem in an open upland field managed by six controlled fertilizer programs using the minimum amount of pesticides. Amplicon sequencing the 16S rRNA gene revealed that inorganic nitrogen fertilizer and compost...
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Published in: | Scientific reports 2024-04, Vol.14 (1), p.9896-9896, Article 9896 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | This study integrated bacterial community and soil chemicals to characterize the soil ecosystem in an open upland field managed by six controlled fertilizer programs using the minimum amount of pesticides. Amplicon sequencing the 16S rRNA gene revealed that inorganic nitrogen fertilizer and compost altered the diversity and structure of the soil bacterial community throughout buckwheat (
Fagopyrum esculentum
Moench ‘Hitachiakisoba’) cultivation. The bacterial community comprised three clusters that contained bacteria that are prevalent in soils fertilized with nitrogen (cluster 1, 340 taxa), without nitrogen and compost (cluster 2, 234 taxa), and with compost-fertilized (cluster 3, 296 taxa). Cluster 2 contained more taxa in
Actinobacteriota
and less in
Acidobacteriota
, and cluster 3 contained more taxa in
Gemmatimonadota
compared with the other clusters. The most frequent taxa in cluster 1 were within the
Chloroflexi
phylum. The bacterial community structure correlated with soil chemical properties including pH, total organic carbon, SO
4
2−
, soluble Ca
2+
. A co-occurrence network of bacterial taxa and chemicals identified key bacterial groups comprising the center of a community network that determined topology and dynamics of the network. Temporal dynamics of the bacterial community structure indicated that
Burkholderiales
were associated with buckwheat ripening, indicating plant-bacteria interaction in the ecosystem. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-024-60655-w |