Recent advances in rational approaches for enzyme engineering

Enzymes are an attractive alternative in the asymmetric syntheses of chiral building blocks. To meet the requirements of industrial biotechnology and to introduce new functionalities, the enzymes need to be optimized by protein engineering. This article specifically reviews rational approaches for e...

Full description

Saved in:
Bibliographic Details
Published in:Computational and structural biotechnology journal 2012-09, Vol.2 (3), p.e201209010-e201209010, Article e201209010
Main Authors: Steiner, Kerstin, Schwab, Helmut
Format: Article
Language:English
Subjects:
artificial metalloenzymes
de novo enzyme design
Mini Review
promiscuous enzymes
protein engineering
rational design
structure-guided engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Enzymes are an attractive alternative in the asymmetric syntheses of chiral building blocks. To meet the requirements of industrial biotechnology and to introduce new functionalities, the enzymes need to be optimized by protein engineering. This article specifically reviews rational approaches for enzyme engineering and de novo enzyme design involving structure-based approaches developed in recent years for improvement of the enzymes' performance, broadened substrate range, and creation of novel functionalities to obtain products with high added value for industrial applications.
ISSN:2001-0370
2001-0370
DOI:10.5936/csbj.201209010