Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b

Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, base...

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Published in:International Journal of Endocrinology 2013-01, Vol.2013 (2013), p.869-879-203
Main Authors: Abe, Tomoki, Kohno, Shohei, Yama, Tomonari, Ochi, Arisa, Suto, Takuro, Hirasaka, Katsuya, Ohno, Ayako, Teshima-Kondo, Shigetada, Okumura, Yuushi, Oarada, Motoko, Choi, Inho, Mukai, Rie, Terao, Junji, Nikawa, Takeshi
Format: Article
Language:English
Subjects:
Allergens
Analysis
Atrophy, Muscular
Diet
Endocrinology
Insulin
Insulin-like growth factors
Laboratory animals
Musculoskeletal system
Peptides
Physiological aspects
Protein synthesis
Proteins
Soybean
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Summary:Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, based on the presence of inhibitory peptides against Cbl-b ubiquitin ligase in soy glycinin protein. Methods. Mice were fed either 20% casein diet, 20% soy protein isolate diet, 10% glycinin diet containing 10% casein, or 20% glycinin diet. One week later, the right sciatic nerve was cut. The wet weight, cross sectional area (CSA), IGF-1 signaling, and atrogene expression in hindlimb muscles were examined at 1, 3, 3.5, or 4 days after denervation. Results. 20% soy glycinin diet significantly prevented denervation-induced decreases in muscle wet weight and myofiber CSA. Furthermore, dietary soy protein inhibited denervation-induced ubiquitination and degradation of IRS-1 in tibialis anterior muscle. Dietary soy glycinin partially suppressed the denervation-mediated expression of atrogenes, such as MAFbx/atrogin-1 and MuRF-1, through the protection of IGF-1 signaling estimated by phosphorylation of Akt-1. Conclusions. Soy glycinin contains a functional inhibitory sequence against muscle-atrophy-associated ubiquitin ligase Cbl-b. Dietary soy glycinin protein significantly prevented muscle atrophy after denervation in mice.
ISSN:1687-8337
1687-8345
DOI:10.1155/2013/907565