Voltage-Gated Ion Channels Are Transcriptional Targets of Sox10 during Oligodendrocyte Development

• Published in: Cells (Basel, Switzerland) Switzerland), 2024-07, Vol.13 (13), p.1159
• Main Authors: Peters, Christian, Aberle, Tim, Sock, Elisabeth, Brunner, Jessica, Küspert, Melanie, Hillgärtner, Simone, Wüst, Hannah M, Wegner, Michael
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Calcium channels (voltage-gated); Cell culture; Cell Differentiation - genetics; Central nervous system; Channel gating; Clonal deletion; Gene Expression Regulation, Developmental; Genes; glia; Glial stem cells; Humans; Hybridization; Invoices; Ion channels; Ion Channels - genetics; Ion Channels - metabolism; Mice; myelin; Neural stem cells; Oligodendrocytes; Oligodendroglia - cytology; Oligodendroglia - metabolism; Physiology; Plasmids; Potassium channels (inwardly-rectifying); Potassium channels (voltage-gated); Progenitor cells; Sodium channels (voltage-gated); Sox protein; Sox10 protein; SOXE Transcription Factors - genetics; SOXE Transcription Factors - metabolism; Spinal cord; Stem cells; Transcription factors; Transcription, Genetic; transcriptional control; Variance analysis
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells13131159

The transcription factor Sox10 is an important determinant of oligodendroglial identity and influences oligodendroglial development and characteristics at various stages. Starting from RNA-seq data, we here show that the expression of several voltage-gated ion channels with known expression and important function in oligodendroglial cells depends upon Sox10. These include the Na 1.1, Ca 2.2, K 1.1, and Kir4.1 channels. For each of the four encoding genes, we found at least one regulatory region that is activated by Sox10 in vitro and at the same time bound by Sox10 in vivo. Cell-specific deletion of Sox10 in oligodendroglial cells furthermore led to a strong downregulation of all four ion channels in a mouse model and thus in vivo. Our study provides a clear functional link between voltage-gated ion channels and the transcriptional regulatory network in oligodendroglial cells. Furthermore, our study argues that Sox10 exerts at least some of its functions in oligodendrocyte progenitor cells, in myelinating oligodendrocytes, or throughout lineage development via these ion channels. By doing so, we present one way in which oligodendroglial development and properties can be linked to neuronal activity to ensure crosstalk between cell types during the development and function of the central nervous system.