Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa PAO1 by Ayurveda spice clove (Syzygium aromaticum) bud extract

Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. viola...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2012-04, Vol.12 (4), p.4016-4030
Main Authors: Krishnan, Thiba, Yin, Wai-Fong, Chan, Kok-Gan
Format: Article
Language:English
Subjects:
Antibiotics
Ayurveda
Ayurvedic medicine
Bacteria
Biofilms
Bioluminescence
Biosensors
Chromobacterium violaceum
E coli
Escherichia coli
Escherichia coli - metabolism
lecA::lux
Luminescence
Motility
N-acylhomoserine lactones
Plant Extracts - pharmacology
Plasmids
Proteobacteria
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - metabolism
Pseudomonas aeruginosa - physiology
pyocyanin
Quorum Sensing - drug effects
Sensors
swarming
Syzygium - chemistry
Syzygium aromaticum
Virulence
Virulence Factors - biosynthesis
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Summary:Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-L-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.
ISSN:1424-8220
1424-8220
DOI:10.3390/s120404016