Single-Cell Atlas Reveals the Hemocyte Subpopulations and Stress Responses in Asian Giant Softshell Turtle during Hibernation

Hibernation in turtle species is an adaptive survival strategy to colder winter conditions or food restrictions. However, the mechanisms underlying seasonal adaptions remain unclear. In the present study, we collected hemocytes from and compared the molecular signature of these cells between the act...

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Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2023-07, Vol.12 (7), p.994
Main Authors: Hong, Xiaoyou, Wang, Yakun, Wang, Kaikuo, Wei, Chengqing, Li, Wei, Yu, Lingyun, Xu, Haoyang, Zhu, Junxian, Zhu, Xinping, Liu, Xiaoli
Format: Article
Language:English
Subjects:
Analysis
Bar codes
Cell differentiation
Cellular stress response
Fishery sciences
Gene expression
Gene mapping
Genes
Genomics
Heat shock
Heat shock proteins
Hematology
Hemocytes
Hibernation
Hsp40 protein
Immune response
Immune system
Immunology
Laboratory animals
Neurosciences
Pelochelys cantorii
Reptiles & amphibians
RNA sequencing
single cell
Software
Stat1 protein
transcriptome
Turtles
Winter
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Summary:Hibernation in turtle species is an adaptive survival strategy to colder winter conditions or food restrictions. However, the mechanisms underlying seasonal adaptions remain unclear. In the present study, we collected hemocytes from and compared the molecular signature of these cells between the active state and hibernation period based on single-cell RNA sequencing (scRNA-seq) analysis. We found six cell types and identified a list of new marker genes for each cell subpopulation. Moreover, several heat shock genes, including the Hsp40 family chaperone gene ( ) and HSP temperature-responsive genes ( ), were upregulated during the hibernation period, which predicted these genes may play crucial roles in the stress response during hibernation. Additionally, compared to hemocytes in the active state, several upregulated differentially expressed immune-related genes, such as , , and , were identified in hemocytes during the hibernation period, thus indicating the important immune function of hemocytes. Therefore, our findings provide a unified classification of . hemocytes and identify the genes related to the stress response, thereby providing a better understanding of the adaptive mechanisms of hibernation.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology12070994