The Role of Swelling in the Regulation of OPA1-Mediated Mitochondrial Function in the Heart In Vitro

Optic atrophy-1 (OPA1) plays a crucial role in the regulation of mitochondria fusion and participates in maintaining the structural integrity of mitochondrial cristae. Here we elucidate the role of OPA1 cleavage induced by calcium swelling in the presence of Myls22 (an OPA1 GTPase activity inhibitor...

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Published in:Cells (Basel, Switzerland) Switzerland), 2023-08, Vol.12 (16), p.2017
Main Authors: Chapa-Dubocq, Xavier R, Rodríguez-Graciani, Keishla M, García-Báez, Jorge, Vadovsky, Alyssa, Bazil, Jason N, Javadov, Sabzali
Format: Article
Language:English
Subjects:
Atrophy
Bioenergetics
Calcium (mitochondrial)
calcium retention capacity
Cellular control mechanisms
Cristae
Depolarization
Experiments
Health aspects
Heart
heart mitochondria
Infrared imaging systems
Laboratory animals
Membrane potential
Membranes
Mitochondria
mitochondrial respiration
mitochondrial swelling
OPA1
Optic atrophy
Physiology
Respiration
Sucrose
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Summary:Optic atrophy-1 (OPA1) plays a crucial role in the regulation of mitochondria fusion and participates in maintaining the structural integrity of mitochondrial cristae. Here we elucidate the role of OPA1 cleavage induced by calcium swelling in the presence of Myls22 (an OPA1 GTPase activity inhibitor) and TPEN (an OMA1 inhibitor). The rate of ADP-stimulated respiration was found diminished by both inhibitors, and they did not prevent Ca2+-induced mitochondrial respiratory dysfunction, membrane depolarization, or swelling. L-OPA1 cleavage was stimulated at state 3 respiration; therefore, our data suggest that L-OPA1 cleavage produces S-OPA1 to maintain mitochondrial bioenergetics in response to stress.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells12162017