Short Communication: Quantification of the Effect of Mycotoxin Binders on the Bioavailability of Fat-Soluble Vitamins In Vitro

• Published in: Animals (Basel) 2021-07, Vol.11 (8), p.2251
• Main Authors: Kihal, Abdelhacib, Rodríguez-Prado, María Ercilda, Cristofol, Carles, Calsamiglia, Sergio
• Format: Article
• Language: English
• Subjects: Activated carbon; Adsorption; Animal feed; Bentonite; Binders; Binding sites; Bioavailability; Calciferol; Cell walls; Communication; Digestion; Essential nutrients; fat-soluble vitamins; Feeds; Incubation; Intestine; Montmorillonite; Mycotoxicosis; mycotoxin binder; Mycotoxins; Nutrients; Retinene; Sepiolite; Tocopherol; Vitamin A; Vitamin D; Vitamin E; Vitamins; Yeast; Yeasts
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani11082251

The aim of this study was to determine the capacity of six mycotoxin binders (MTBs) to adsorb vitamins A, D and E in an in vitro system that simulates gastric and intestinal digestion. Experiment 1 evaluated the recovery rate of vitamins A, D and E in the incubation conditions. In Experiment 2, the main factors were the MTB (bentonite, clinoptilolite, sepiolite, montmorillonite, active carbon and yeast cell walls), vitamins (A, D and E) and incubation type (vitamins incubated separately or together). The recovery was high for vitamin D (83%) and E (93%), but low for vitamin A (23%), for which no further analyses were conducted. When incubated separately, vitamin D was only adsorbed by yeast cell wall (20.2%). Vitamin E adsorption was highest with bentonite (54.5%) and montmorillonite (46.3%) and lowest with sepiolite (16.6%) and active carbon (18.5%). When incubated together, vitamin D was not adsorbed by any MTB. Vitamin E adsorption was highest in bentonite (61.8%) and montmorillonite (50.7%) and lowest in sepiolite (15.4%). Results indicate that the bioavailability of vitamin E, but not that of vitamin D, may be reduced in the presence of MTBs.