MiR-18a affects hypoxia induced glucose metabolism transition in HT22 hippocampal neuronal cell line through the Hif1a gene

• Published in: BMC neurology 2024-06, Vol.24 (1), p.204-12
• Main Authors: Liu, Chuncheng, Liu, Gehui, Zuo, Xinyang, Qu, Donghui, Sun, Yefeng, Liu, Linan, Zhao, Xiujuan, Li, Jun, Cai, Lu
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Acids; Angiogenesis; Animals; Breast cancer; Cardiovascular disease; Cell culture; Cell Hypoxia - physiology; Cell Line; Central nervous system; Chloride; Cloning; Cobalt; Dextrose; Genes; Glucose; Glucose - deficiency; Glucose - metabolism; Glycolysis; Hif1a; Hippocampus; Hippocampus - metabolism; Hippocampus - pathology; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Hypoxia-inducible factors; Ischemia; Medical research; Medicine, Experimental; Metabolism; Metastasis; Mice; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miR-18a; Nervous system; Nervous system diseases; Neurodegenerative diseases; Neuronal; Neurons; Neurons - metabolism; Oxygen; Polymerase chain reaction; Proteins; Statistical analysis; Stroke (Disease)
• ISSN: 1471-2377; 1471-2377
• DOI: 10.1186/s12883-024-03717-w

Hypoxia can cause a variety of diseases, including ischemic stroke and neurodegenerative diseases. Within a certain range of partial pressure of oxygen, cells can respond to changes in oxygen. Changes in oxygen concentration beyond a threshold will cause damage or even necrosis of tissues and organs, especially for the central nervous system. Therefore, it is very important to find appropriate measures to alleviate damage. MiRNAs can participate in the regulation of hypoxic responses in various types of cells. MiRNAs are involved in regulating hypoxic responses in many types of tissues by activating the hypoxia-inducible factor (HIF) to affect angiogenesis, glycolysis and other biological processes. By analyzing differentially expressed miRNAs in hypoxia and hypoxia-related studies, as well as the HT22 neuronal cell line under hypoxic stress, we found that the expression of miR-18a was changed in these models. MiR-18a could regulate glucose metabolism in HT22 cells under hypoxic stress by directly regulating the 3'UTR of the Hif1a gene. As a small molecule, miRNAs are easy to be designed into small nucleic acid drugs, so this study can provide a theoretical basis for the research and treatment of nervous system diseases caused by hypoxia.