The mouse multi-organ proteome from infancy to adulthood

• Published in: Nature communications 2024-07, Vol.15 (1), p.5752-14, Article 5752
• Main Authors: Wang, Qingwen, Ding, Xinwen, Xu, Zhixiao, Wang, Boqian, Wang, Aiting, Wang, Liping, Ding, Yi, Song, Sunfengda, Chen, Youming, Zhang, Shuang, Jiang, Lai, Ding, Xianting
• Format: Article
• Language: English
• Subjects: 631/136/2060; 631/1647/296; 631/45/475; 64/60; 82/29; 82/58; 82/80; Age; Animals; Brain - growth & development; Brain - metabolism; Data acquisition; Deep learning; Developmental biology; Developmental stages; Female; Gene Expression Regulation, Developmental; Heart; Humanities and Social Sciences; Intestine; Kidney - growth & development; Kidney - metabolism; Laboratories; Liver; Liver - metabolism; Lung - growth & development; Lung - metabolism; Male; Mass spectrometry; Mass spectroscopy; Maturation; Mice; Mice, Inbred C57BL; Molecular modelling; multidisciplinary; Organ Specificity; Organs; Phenotypes; Proteins; Proteome - metabolism; Proteomes; Proteomics; Proteomics - methods; Science; Science (multidisciplinary); Scientific imaging; Sex Characteristics; Sexual dimorphism; Spleen; Spleen - growth & development; Spleen - metabolism; Spliceosomes - metabolism; Stomach
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-50183-6

The early-life organ development and maturation shape the fundamental blueprint for later-life phenotype. However, a multi-organ proteome atlas from infancy to adulthood is currently not available. Herein, we present a comprehensive proteomic analysis of ten mouse organs (brain, heart, lung, liver, kidney, spleen, stomach, intestine, muscle and skin) at three crucial developmental stages (1-, 4- and 8-weeks after birth) acquired using data-independent acquisition mass spectrometry. We detect and quantify 11,533 protein groups across the ten organs and obtain 115 age-related differentially expressed protein groups that are co-expressed in all organs from infancy to adulthood. We find that spliceosome proteins prevalently play crucial regulatory roles in the early-life development of multiple organs, and detect organ-specific expression patterns and sexual dimorphism. This multi-organ proteome atlas provides a fundamental resource for understanding the molecular mechanisms underlying early-life organ development and maturation. Multi-organ proteomic data is needed to understand the complex processes of early-life organ development and maturation. Here, the authors generated a proteomic atlas covering the development of ten mouse organs from infancy to adulthood and report organ- and age-specific signaling pathways and co-expressed proteins.