Fiber Protein Produced in Escherichia coli as a Subunit Vaccine Candidate Against Egg-Drop Syndrome 76

The egg-drop syndrome '76 (EDS '76) caused by duck atadenovirus A (DAdV-1) infection in laying hens leads to the decrease in egg production, causing heavy economic losses in the poultry industry; thus, vaccines with high safety and immunogenicity are needed. In this study, the DAdV-1 fiber...

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Bibliographic Details
Published in:Frontiers in veterinary science 2022-02, Vol.9, p.819217-819217
Main Authors: Wang, Linguo, Zhang, Pantao, Huang, Baicheng, Wang, Mengyue, Tian, Hui, Liu, Peng, Liu, Wujie, Tian, Kegong
Format: Article
Language:English
Subjects:
duck atadenovirus A
Escherichia coli
fiber protein
immunogenicity
subunit vaccine candidate
Veterinary Science
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Summary:The egg-drop syndrome '76 (EDS '76) caused by duck atadenovirus A (DAdV-1) infection in laying hens leads to the decrease in egg production, causing heavy economic losses in the poultry industry; thus, vaccines with high safety and immunogenicity are needed. In this study, the DAdV-1 fiber protein expressed in with codon optimization showed the hemagglutination (HA) titer of 13 log2 after purification (0.6 mg/mL). Compared with inactivated EDS '76 vaccine, the specific pathogen-free chickens immunized with 0.4 mL fiber protein (HA titer of 11 log2) induced an equal level of HA inhibition (HI) titer and neutralizing antibodies. Meanwhile, after immunization with fiber protein, the lowest HI titer that could provide the effect to reduce egg production rate in laying hens after the challenge was 7 log2. Moreover, fiber protein with an HA titer of 7 log2 could induce an HI titer no
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2022.819217