Small G Protein StRab5b positively regulates potato resistance to Phytophthora infestans

Rabproteins are the largest members of the small G protein family and are widely distributed in eukaryotes. It comprises eight subfamilies and is responsible for regulating vesicle transport, plant growth and development, and biotic and abiotic stress responses. In this study, the small G protein ge...

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Published in:Frontiers in plant science 2023-01, Vol.13, p.1065627-1065627
Main Authors: Tian, Zaimin, Zhang, Zhiwei, Kang, Liru, Li, Min, Zhang, Jian, Feng, Yan, Yin, Jiang, Gong, Xuechen, Zhao, Jun
Format: Article
Language:English
Subjects:
antioxidant enzyme activities
gene expression
hormone signaling pathways
plant disease resistance
Plant Science
potato late blight
Small G proteins
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Summary:Rabproteins are the largest members of the small G protein family and are widely distributed in eukaryotes. It comprises eight subfamilies and is responsible for regulating vesicle transport, plant growth and development, and biotic and abiotic stress responses. In this study, the small G protein gene was cloned from potato, and its biological information, expression profile and induced expression level, overexpression and gene silencing were examined on regulating potato resistance to using PCR, qPCR and Virus-induced gene silencing (VIGS). Our results indicate that the amino acid of StRab5b shows the highest and lowest homology with NbRab5b in and StRab in potato respectively. expression varied among different potato tissues and varieties, and was induced by infection. Transiently ectopic expression of in enhanced its resistance to , whereas, silencing of and its homologous gene facilitated pathogen infection in potato and respectively. Furthermore, stable expression of the gene in potatoes enhanced its redox-stress response capacity, as manifested by the accumulation of H O in infected leaves and subsequent increase in the activity and expression of ROS scavenging enzymes, thereby attenuating the development of and ultimately reducing the lesions on infected potato leaves. In addition, the gene transcripts and JA level were upregulated rapidly after inoculation with . Collectively, our results suggest that positively regulates the resistance against potato late blight (PLB) JA-mediated defense signaling pathway.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.1065627