Protein-mediated palmitate uptake and expression of fatty acid transport proteins in heart giant vesicles

Giant sarcolemmal vesicles were isolated from rat heart and hindlimb muscles for a) characterization of long-chain fatty acid transport in the absence of metabolism and b) comparison of fatty acid transport protein expression with fatty acid transport. Giant vesicles contained cytosolic fatty acid b...

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Bibliographic Details
Published in:Journal of lipid research 1999-06, Vol.40 (6), p.1007-1016
Main Authors: Luiken, J J, Turcotte, L P, Bonen, A
Format: Article
Language:English
Subjects:
Animals
Biological Transport - drug effects
Carrier Proteins - metabolism
CD36 Antigens
FABPpm
FAT/CD36
FATP
Fatty Acid-Binding Protein 7
Fatty Acid-Binding Proteins
Hindlimb
Immune Sera - pharmacology
Kinetics
Membrane Glycoproteins - metabolism
muscle
Muscle, Skeletal - ultrastructure
Myelin P2 Protein - metabolism
Myocardium - ultrastructure
Neoplasm Proteins
Nerve Tissue Proteins
Organic Anion Transporters
Palmitic Acid - metabolism
Phloretin - pharmacology
Rats
Sarcolemma - metabolism
Trypsin - pharmacology
uptake kinetics
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Summary:Giant sarcolemmal vesicles were isolated from rat heart and hindlimb muscles for a) characterization of long-chain fatty acid transport in the absence of metabolism and b) comparison of fatty acid transport protein expression with fatty acid transport. Giant vesicles contained cytosolic fatty acid binding protein. Palmitate uptake was completely divorced from its metabolism. All palmitate taken up was recovered in the intravesicular cytosol as unesterified FA. Palmitate uptake by heart vesicles exhibited a K m of 9.7 nm, similar to that of muscle (K m = 9.7 nm). Vmax (2.7 pmol/mg protein/s) in heart was 8-fold higher than in muscle (0.34 pmol/mg protein/s). Palmitate uptake was inhibited in heart (55-80%) and muscle (31-50%) by trypsin, phloretin, sulfo-N-succinimidyloleate (SSO), or a polyclonal antiserum against the 40 kDa plasma membrane fatty acid binding protein (FABPpm). Palmitate uptake by heart and by red and white muscle vesicles correlated well with the expression of fatty acid translocase (FAT/CD36) and fatty acid binding protein FABPpm, which may act in concert. The expression of fatty acid transport protein (FATP), was 10-fold lower in heart vesicles than in white muscle vesicles. It is concluded that long-chain fatty acid uptake by heart and muscle vesicles is largely protein-mediated, involving FAT/CD36 and FABPpm. The role of FATP in muscle and heart remains uncertain.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)33504-5