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DNA intercalator stimulates influenza transcription and virus replication
Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA tr...
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| Published in: | Virology journal 2011-03, Vol.8 (1), p.120-120, Article 120 |
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| container_title | Virology journal |
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| creator | Li, Olive T W Poon, Leo L M |
| description | Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII(a) in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII(a)) to hyperphosphorylated RNAPII (RNAPII(o)). |
| doi_str_mv | 10.1186/1743-422X-8-120 |
| format | article |
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In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII(a) in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII(a)) to hyperphosphorylated RNAPII (RNAPII(o)).</description><identifier>ISSN: 1743-422X</identifier><identifier>EISSN: 1743-422X</identifier><identifier>DOI: 10.1186/1743-422X-8-120</identifier><identifier>PMID: 21401962</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Cell Line ; Clathrate compounds ; Cyclin-dependent kinases ; Dactinomycin - pharmacology ; Experiments ; Gene expression ; Gene Expression Regulation, Viral - drug effects ; Genetic aspects ; Genetic transcription ; Humans ; Infections ; Influenza ; Influenza A virus - drug effects ; Influenza A virus - enzymology ; Influenza A virus - genetics ; Influenza A virus - physiology ; Influenza viruses ; Influenza, Human - virology ; Intercalating Agents - pharmacology ; Kinases ; Phosphorylation ; Plasmids ; Proteins ; RNA Polymerase II - antagonists & inhibitors ; RNA Polymerase II - genetics ; RNA Polymerase II - metabolism ; Short Report ; Transcription, Genetic - drug effects ; Viral infections ; Viral Proteins - antagonists & inhibitors ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Virus Replication - drug effects ; Viruses</subject><ispartof>Virology journal, 2011-03, Vol.8 (1), p.120-120, Article 120</ispartof><rights>COPYRIGHT 2011 BioMed Central Ltd.</rights><rights>2011 Li and Poon; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2011 Li and Poon; licensee BioMed Central Ltd. 2011 Li and Poon; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-b599t-cc7c3305aa942f89a5aba848922c36efbd2b922e609544b31f76d43098272c1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069954/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/902295897?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21401962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Olive T W</creatorcontrib><creatorcontrib>Poon, Leo L M</creatorcontrib><title>DNA intercalator stimulates influenza transcription and virus replication</title><title>Virology journal</title><addtitle>Virol J</addtitle><description>Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII(a) in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII(a)) to hyperphosphorylated RNAPII (RNAPII(o)).</description><subject>Animals</subject><subject>Cell Line</subject><subject>Clathrate compounds</subject><subject>Cyclin-dependent kinases</subject><subject>Dactinomycin - pharmacology</subject><subject>Experiments</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Viral - drug effects</subject><subject>Genetic aspects</subject><subject>Genetic transcription</subject><subject>Humans</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza A virus - drug effects</subject><subject>Influenza A virus - enzymology</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - physiology</subject><subject>Influenza viruses</subject><subject>Influenza, Human - virology</subject><subject>Intercalating Agents - pharmacology</subject><subject>Kinases</subject><subject>Phosphorylation</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>RNA Polymerase II - antagonists & inhibitors</subject><subject>RNA Polymerase II - genetics</subject><subject>RNA Polymerase II - metabolism</subject><subject>Short Report</subject><subject>Transcription, Genetic - drug effects</subject><subject>Viral infections</subject><subject>Viral Proteins - antagonists & inhibitors</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Virus Replication - drug effects</subject><subject>Viruses</subject><issn>1743-422X</issn><issn>1743-422X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp1kstv1DAQxiMEog84c0MRnHpIa4-dxL4gLeW1UgUSD4mbNXGcxask3tpOBfz1OGxZNVKRDx598_mneTjLnlFyTqmoLmjNWcEBvheioEAeZMcH5eGd-Cg7CWFLCIOqlo-zI6CcUFnBcbZ-83GV2zEar7HH6Hweoh2mFJqQ9K6fzPgb8-hxDNrbXbRuzHFs8xvrp5B7s-utxll9kj3qsA_m6e19mn179_br5Yfi6tP79eXqqmhKKWOhda0ZIyWi5NAJiSU2KLiQAJpVpmtaaFJsKiJLzhtGu7pqOSNSQA2aanaarffc1uFW7bwd0P9SDq36Kzi_Ueij1b1RpGpbIBoQiUks3ghidAlJLYE3dZlYr_as3dQMptVmTI32C-gyM9ofauNuFCOVTPUlwOs9oLHuP4BlRrtBzWtR81qUUGlpCfLitgrvricTotq6yY9piEoSAFkKWSfTy71pg6mztBmXeHqwQasVlCBFDXR2nd_jSqc1g9VuNJ1N-uLB2eJB8kTzM25wCkGtv3xeei_2Xu1dCN50hz4pUfN3vKez53fne_D_-3_sD4y22jg</recordid><startdate>20110315</startdate><enddate>20110315</enddate><creator>Li, Olive T W</creator><creator>Poon, Leo L M</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110315</creationdate><title>DNA intercalator stimulates influenza transcription and virus replication</title><author>Li, Olive T W ; Poon, Leo L M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b599t-cc7c3305aa942f89a5aba848922c36efbd2b922e609544b31f76d43098272c1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>Clathrate compounds</topic><topic>Cyclin-dependent kinases</topic><topic>Dactinomycin - pharmacology</topic><topic>Experiments</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Viral - drug effects</topic><topic>Genetic aspects</topic><topic>Genetic transcription</topic><topic>Humans</topic><topic>Infections</topic><topic>Influenza</topic><topic>Influenza A virus - drug effects</topic><topic>Influenza A virus - enzymology</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - physiology</topic><topic>Influenza viruses</topic><topic>Influenza, Human - virology</topic><topic>Intercalating Agents - pharmacology</topic><topic>Kinases</topic><topic>Phosphorylation</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>RNA Polymerase II - antagonists & inhibitors</topic><topic>RNA Polymerase II - genetics</topic><topic>RNA Polymerase II - metabolism</topic><topic>Short Report</topic><topic>Transcription, Genetic - drug effects</topic><topic>Viral infections</topic><topic>Viral Proteins - antagonists & inhibitors</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Virus Replication - drug effects</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Olive T W</creatorcontrib><creatorcontrib>Poon, Leo L M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Virology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Olive T W</au><au>Poon, Leo L M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA intercalator stimulates influenza transcription and virus replication</atitle><jtitle>Virology journal</jtitle><addtitle>Virol J</addtitle><date>2011-03-15</date><risdate>2011</risdate><volume>8</volume><issue>1</issue><spage>120</spage><epage>120</epage><pages>120-120</pages><artnum>120</artnum><issn>1743-422X</issn><eissn>1743-422X</eissn><abstract>Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII). In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral RNA transcription. A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication. This effect was not observed in cells treated with RNAPII kinase inhibitors. In addition, the loss of RNAPII(a) in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPII(a)) to hyperphosphorylated RNAPII (RNAPII(o)).</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>21401962</pmid><doi>10.1186/1743-422X-8-120</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Virology journal, 2011-03, Vol.8 (1), p.120-120, Article 120 |
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| subjects | Animals Cell Line Clathrate compounds Cyclin-dependent kinases Dactinomycin - pharmacology Experiments Gene expression Gene Expression Regulation, Viral - drug effects Genetic aspects Genetic transcription Humans Infections Influenza Influenza A virus - drug effects Influenza A virus - enzymology Influenza A virus - genetics Influenza A virus - physiology Influenza viruses Influenza, Human - virology Intercalating Agents - pharmacology Kinases Phosphorylation Plasmids Proteins RNA Polymerase II - antagonists & inhibitors RNA Polymerase II - genetics RNA Polymerase II - metabolism Short Report Transcription, Genetic - drug effects Viral infections Viral Proteins - antagonists & inhibitors Viral Proteins - genetics Viral Proteins - metabolism Virus Replication - drug effects Viruses |
| title | DNA intercalator stimulates influenza transcription and virus replication |
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