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Exome sequencing revealed comparable frequencies of RNF43 and BRAF mutations in Middle Eastern colorectal cancer
Mutation-induced activation of Wnt -β Catenin signaling pathway is frequent in CRC. The E3 ubiquitin ligase, RNF43 , has been reported to negatively regulate the Wnt signaling pathway and RNF43 mutations are frequently seen in CRC. However, its role in Middle Eastern CRC remains unclear. Therefore,...
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Published in: | Scientific reports 2022-07, Vol.12 (1), p.13098-13098, Article 13098 |
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Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Mutation-induced activation of
Wnt
-β Catenin signaling pathway is frequent in CRC. The E3 ubiquitin ligase,
RNF43
, has been reported to negatively regulate the
Wnt
signaling pathway and
RNF43
mutations are frequently seen in CRC. However, its role in Middle Eastern CRC remains unclear. Therefore, we employed Exome and Sanger sequencing technology to assess the frequency of
RNF43
mutations and its association with other clinico-pathological features in Middle Eastern CRC.
RNF43
mutations were found in 5.9% (13/220) of CRC cases and was inversely correlated to
APC
and
TP53
mutations. A strong association of
RNF43
mutations with right sided and sporadic microsatellite instable (MSI) CRC was observed. No association was identified between
RNF43
mutation and other clinico-pathological features including
BRAF
mutation, age, tumor histological subtype, tumor grade or patients’ prognosis. Multivariate logistic regression analysis revealed that MSI status and wild type
APC
were independent predictor of
RNF43
mutation. We conclude that
RNF43
mutations occur in Middle Eastern CRC at comparable frequencies with
BRAF
mutations and represent a distinct molecular subtype which further enhances our understanding of how different mutational subsets of
Wnt
tumor suppressor genes link to distinct tumor characteristics, which might be considered for treatment strategies for CRC patients. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-022-17449-9 |