Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for point-of-care detection of SARS-CoV-2: a clinical study to evaluate agreement with RT-qPCR

The global COVID-19 pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the need for rapid, accurate, and accessible diagnostics to enable timely treatment and outbreak control. However, current diagnostic tests based on RT-qPCR are insufficient...

Full description

Saved in:
Bibliographic Details
Published in:The Lancet global health 2021-03, Vol.9, p.S3-S3
Main Authors: Natoli, Mary, Kundrod, Kathryn, Chang, Megan, Smith, Chelsey, Paul, Sai, Eldin, Karen, Patel, Keyur, Baker, Ellen, Schmeler, Kathleen, Richards-Kortum, Rebecca
Format: Article
Language:English
Subjects:
Abstracts
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The global COVID-19 pandemic caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted the need for rapid, accurate, and accessible diagnostics to enable timely treatment and outbreak control. However, current diagnostic tests based on RT-qPCR are insufficient to meet the global testing demand because of their high cost and complexity and supply chain shortages. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a promising alternative to RT-qPCR because of its sensitivity, speed, and robustness to sample inhibitors. Here, we describe the development and optimisation of a sample-to-answer workflow, including a simple lysis and inactivation protocol that provides results in
ISSN:2214-109X
2214-109X
DOI:10.1016/S2214-109X(21)00111-X