Effects of Dietary Supplementation with Aurantiochytrium sp. on Zebrafish Growth as Determined by Transcriptomics

• Published in: Animals (Basel) 2022-10, Vol.12 (20), p.2794
• Main Authors: Yang, Hao, Huang, Yanlin, Li, Zhiyuan, Guo, Yuwen, Li, Shuangfei, Huang, Hai, Yang, Xuewei, Li, Guangli, Chen, Huapu
• Format: Article
• Language: English
• Subjects: Acetyl-CoA carboxylase; Aquaculture; Aurantiochytrium sp; Carnitine; Cell proliferation; Danio rerio; Docosahexaenoic acid; Eicosapentaenoic acid; Encyclopedias; Enzymatic activity; Enzyme activity; Enzymes; Experiments; Fatty acids; Fatty-acid synthase; Fish meal; Fish oils; Gene expression; Genes; Genomes; Glucose 6 phosphate dehydrogenase; Glucosephosphate dehydrogenase; Glutathione peroxidase; growth; Hepatocytes; Kinases; Laboratory animals; Lipase; Lipid metabolism; Lipid peroxidation; Lipids; Liver; Malic enzyme; metabolic enzymes; Oxidation; Peroxidase; Peroxidation; Signal transduction; Superoxide dismutase; transcriptome; Transcriptomes; Transcriptomics; Zebrafish
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani12202794

The marine protist Aurantiochytrium produces several bioactive chemicals, including EPA (eicosapentaenoic acid), DHA (docosahexaenoic acid), and other critical fish fatty acids. It has the potential to improve growth and fatty acid profiles in aquatic taxa. This study evaluated zebrafish growth performance in response to diets containing 1% to 3% Aurantiochytrium sp. crude extract (TE) and single extract for 56 days. Growth performance was best in the 1% TE group, and therefore, this concentration was used for further analyses of the influence of Aurantiochytrium sp. Levels of hepatic lipase, glucose-6-phosphate dehydrogenase, acetyl-CoA oxidase, glutathione peroxidase, and superoxide dismutase increased significantly in response to 1% TE, while malic enzyme activity, carnitine lipid acylase, acetyl-CoA carboxylase, fatty acid synthase, and malondialdehyde levels decreased. These findings suggest that Aurantiochytrium sp. extract can modulate lipase activity, improve lipid synthesis, and decrease oxidative damage caused by lipid peroxidation. Transcriptome analysis revealed 310 genes that were differentially expressed between the 1% TE group and the control group, including 185 up-regulated genes and 125 down-regulated genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway analyses of the differentially expressed genes revealed that Aurantiochytrium sp. extracts may influence liver metabolism, cell proliferation, motility, and signal transduction in zebrafish.