Identification and Functional Analysis of Glutathione S-Transferases from Sitophilus zeamais in Olfactory Organ

Odorant-degrading enzymes (ODEs) play an important role in rapidly degrading and inactivating odorant molecules that have completed information transmission, as well as in maintaining the stability and sensitivity of insect olfactory sensing systems. Glutathione S-transferases (GSTs), as a group of...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2022-03, Vol.13 (3), p.259
Main Authors: Xia, Daosong, Zheng, Renwen, Huang, Jianhua, Lu, Sihan, Tang, Qingfeng
Format: Article
Language:English
Subjects:
Amino acids
Antennae
Biological control
Conjugation
Degradation
degradation metabolism
Enzymes
Functional analysis
Genes
Glutathione
glutathione S-transferases
Host location
Information processing
Insects
Metabolites
odorant-degrading enzymes
Olfaction
Olfactory organs
Pattern analysis
Pesticides
Proteins
Sitophilus zeamais
Substrates
Volatiles
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Summary:Odorant-degrading enzymes (ODEs) play an important role in rapidly degrading and inactivating odorant molecules that have completed information transmission, as well as in maintaining the stability and sensitivity of insect olfactory sensing systems. Glutathione S-transferases (GSTs), as a group of ODEs, supposedly bear the ability to catalyze the conjugation of glutathione (GSH) and xenobiotic odorant molecules in the degrading process. However, there are few reports regarding the role of the GST genes of in the degrading process. Thus, we characterized 13 full-length genes encoding GST sequences from , of which only contained a high abundance in the antennae. Ligand-binding assays implied that SzeaGSTd1 was able to catalyze the conjugation of GSH with 2, 4-dinitrochlorobenzene (CDNB). We investigated whether recombinant SzeaGSTd1 bears the ability to degrade the volatile molecules of the host; among the host volatiles, and found capryl alcohol to be a suitable substrate for SzeaGSTd1. These results strongly suggest that SzeaGSTd1 probably plays a role in auxiliary host location by degrading the host volatiles of capryl alcohol and exhibits a potential biological function in the olfactory sensing system of . Knowledge of the potential functions of SzeaGSTd1 will provide new ideas for biological control strategies for .
ISSN:2075-4450
2075-4450
DOI:10.3390/insects13030259