CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression

Chromatin accessibility plays important roles in revealing the regulatory networks of gene expression, while its application in bladder cancer is yet to be fully elucidated. Chloride intracellular channel 3 (CLIC3) protein has been reported to be associated with the progression of some tumors, where...

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Bibliographic Details
Published in:Cell death & disease 2024-01, Vol.15 (1), p.9-9, Article 9
Main Authors: Shuai, Yujun, Zhang, Hui, Liu, Changhao, Wang, Junting, Jiang, Yangkai, Sun, Jiayin, Gao, Xincheng, Bo, Xiaochen, Xiao, Xingyuan, Liao, Xin, Huang, Chao, Chen, Hebing, Jiang, Guosong
Format: Article
Language:English
Subjects:
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Antibodies
Biochemistry
Biomedical and Life Sciences
Bladder cancer
Cell Biology
Cell Culture
Cell proliferation
Chloride Channels - genetics
Chromatin
Cyclin-dependent kinase inhibitor p21
Gene expression
Humans
Immunology
Life Sciences
mRNA stability
N-Terminal Acetyltransferases
RNA modification
RNA, Messenger - genetics
Therapeutic targets
Urinary Bladder
Urinary Bladder Neoplasms - genetics
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Summary:Chromatin accessibility plays important roles in revealing the regulatory networks of gene expression, while its application in bladder cancer is yet to be fully elucidated. Chloride intracellular channel 3 (CLIC3) protein has been reported to be associated with the progression of some tumors, whereas the specific mechanism of CLIC3 in tumor remains unclear. Here, we screened for key genes in bladder cancer through the identification of transcription factor binding site clustered region (TFCR) on the basis of chromatin accessibility and TF motif. CLIC3 was identified by joint profiling of chromatin accessibility data with TCGA database. Clinically, CLIC3 expression was significantly elevated in bladder cancer and was negatively correlated with patient survival. CLIC3 promoted the proliferation of bladder cancer cells by reducing p21 expression in vitro and in vivo. Mechanistically, CLIC3 interacted with NAT10 and inhibited the function of NAT10, resulting in the downregulation of ac4C modification and stability of p21 mRNA. Overall, these findings uncover an novel mechanism of mRNA ac4C modification and CLIC3 may act as a potential therapeutic target for bladder cancer.
ISSN:2041-4889
2041-4889
DOI:10.1038/s41419-023-06373-z