CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis

In the present study we have investigated the likely impact of oral vaccination of weaned pigs with non-enterotoxigenic Escherichia coli strain expressing Fac antigen on CD45RA and CD45RC isoforms expression on the surface of the mesenteric lymph node (MLN) cells and on spleen cells by using one-col...

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Bibliographic Details
Published in:Veterinární medicína 2002-01, Vol.47 (1), p.5-11
Main Authors: Bozic, F. (University of Zagreb (Croatia). Dept. of Pharmacology and Toxicology), Sver, L, Valpotic, I
Format: Article
Language:English
Subjects:
Animal behavior
ANTIGENE
ANTIGENOS
ANTIGENS
BAZO
cd25
CD25 antigen
cd45 isoforms
CD45RA antigen
Cell activation
CELL COUNTING
Colibacillosis
CONTEO DE CELULAS
DIARREA
Diarrhea
DIARRHEE
DIARRHOEA
E coli
ESCHERICHIA COLI
flow cytom
Flow cytometry
Immunoprophylaxis
Isoforms
LECHON
LINFOCITOS
LIVE VACCINES
Lymph nodes
LYMPHATIC SYSTEM
LYMPHOCYTE
LYMPHOCYTES
Lymphocytes T
Morbidity
NUMERATION CELLULAIRE
PERIODE APRES SEVRAGE
PERIODO POSTDESTETE
Phenotypes
PIGLETS
PORCELET
POSTWEANING PERIOD
RATE
SISTEMA LINFATICO
SPLEEN
Swine
SYSTEME LYMPHATIQUE
VACCIN VIVANT
VACCINATION
Vaccines
VACUNA VIVA
VACUNACION
weaned pigs
Weaning
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Summary:In the present study we have investigated the likely impact of oral vaccination of weaned pigs with non-enterotoxigenic Escherichia coli strain expressing Fac antigen on CD45RA and CD45RC isoforms expression on the surface of the mesenteric lymph node (MLN) cells and on spleen cells by using one-colour flow cytometry. Additionally, to assess the activation state of T cells in the MLN and spleen of the pigs, surface expression of the lymphocyte activation marker CD25 was analysed. Sham-vaccinated weaned pigs served as controls. Our results of the quantitative phenotypic analysis of isolated lymphocytes showed that the vaccinal E. coli strain induced elevation of both CD25+ and CD45RC+ cells in the spleen, but not MLN. This was accompanied by decreased CD45RA expression on spleen cells, suggesting that CD45RA+ spleen cells could develop CD45RC phenotype, probably as a consequence of activation in the vaccinated challenge-infected weaned pigs.
ISSN:0375-8427
1805-9392
DOI:10.17221/5795-VETMED