A 48-bp deletion upstream of LIGULELESS 1 alters rice panicle architecture

Panicle architecture is an agronomic determinant of crop yield and a target for cereal crop improvement. To investigate its molecular mechanisms in rice, we performed map-based cloning and characterization of OPEN PANICLE 1 (OP1), a gain-of-function allele of LIGULELESS 1 (LG1), controlling the spre...

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Bibliographic Details
Published in:The Crop journal 2024-04, Vol.12 (2), p.354-363
Main Authors: Wu, Linhua, Hu, Min, Lyu, Shuwei, Chen, Wenfeng, Yu, Hang, Liu, Qing, He, Wei, Li, Chen, Zhu, Zuofeng
Format: Article
Language:English
Subjects:
Agricultural production
Agronomic crops
Alleles
Barley
brassinosteroids
Cereal crops
Cloning
Corn
Crop improvement
Crop yield
Deletion
Domestication
gain-of-function mutation
Gene deletion
Gene expression
Genes
genetically modified organisms
Genomes
Genotype & phenotype
Localization
Molecular modelling
Mutants
Panicle architecture
panicles
phenotype
Phenotypes
Polymerase chain reaction
pulvinus
Rice
Signal transduction
Transgenic plants
Upstream region
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Summary:Panicle architecture is an agronomic determinant of crop yield and a target for cereal crop improvement. To investigate its molecular mechanisms in rice, we performed map-based cloning and characterization of OPEN PANICLE 1 (OP1), a gain-of-function allele of LIGULELESS 1 (LG1), controlling the spread-panicle phenotype. This allele results from a 48-bp deletion in the LG1 upstream region and promotes pulvinus development at the base of the primary branch. Increased OP1 expression and altered panicle phenotype in chimeric transgenic plants and upstream-region knockout mutants indicated that the deletion regulates spread-panicle architecture in the mutant spread panicle 1 (sp1). Knocking out BRASSINOSTEROID UPREGULATED1 (BU1) gene in the background of OP1 complementary plants resulted in compact panicles, suggesting OP1 may regulate inflorescence architecture via the brassinosteroid signaling pathway. We regard that manipulating the upstream regulatory region of OP1 or genes involved in BR signal pathway could be an efficient way to improve rice inflorescence architecture.
ISSN:2214-5141
2095-5421
2214-5141
DOI:10.1016/j.cj.2023.12.008