Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt

• Published in: Veterinary World 2020-07, Vol.13 (7), p.1473-1479
• Main Authors: El-Shannat, Sara M, El-Tawab, Ashraf A Abd, Hassan, Wafaa M M
• Format: Article
• Language: English
• Subjects: analytical profile index 20e; Antibiotics; Foodborne diseases; Frankfurters; Gram-negative bacteria; Ions; Mass spectroscopy; matrix-assisted laser-desorption ionization time-of-flight mass spectrometry; Meat; Multidrug resistance; Pathogens; phenotypic system; Phylogeny; Raoultella ornithinolytica
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.1473-1479

is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between and are difficult, as they are phylogenetic related. The evolution of multidrug resistance of strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of , isolated from chicken product samples, and assessed the resistance profile of . using antibiogram sensitivity tests. Forty samples of chicken products were collected between January and September 2019 from different markets in Alexandria Governorate, Egypt. The products included nuggets, strips, burgers, luncheon meats, pane, frankfurters, and minced chicken meat. The samples were transferred to the Reference Laboratory. The samples were subjected to isolation, biochemical reaction testing, phenotypic system analytical profile index (API) E20, and a detection of antimicrobial susceptibility test. Phenotypic identification was confirmed through matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Thirty-three bacterial isolates (82.50%) out of 40 samples were isolated into pure cultures from the chicken samples. Three isolates (9.09%) were positive for , while 30 isolates (90.91%) exhibited growth characters for different pathogens ( , , , and ). The isolates of were resistant to five types of antibiotics and sensitive to two types of antibiotics. This study reported the first case of found in chicken products in Egypt. Phenotypic system API 20E and MALDI-TOF MS were found to be reliable tools for confirming the diagnosis of a. As it provides rapid identification with high sensitivity and specificity for , which often do not require a molecular procedure for confirmation.