Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor

Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5vvm increased the yield by 16.6% (from 13.81 to 16.1g/l) upon controlling the temperature at 28°C. Adjustment of the aeration level at 1.5v...

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Bibliographic Details
Published in:Bulletin of the Faculty of Pharmacy 2013-06, Vol.51 (1), p.43-48
Main Authors: El-Sedawy, Hewaida F., Hussein, Manal M.M., Essam, Tamer, El-Tayeb, Ossama M., Mohammad, Fatma H.A.
Format: Article
Language:English
Subjects:
Amycolatopsis mediterranei
Antibiotics
Fermentor
Rifamycin B
Scaling up
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Summary:Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH) was carried out. The use of aeration level 1.5vvm increased the yield by 16.6% (from 13.81 to 16.1g/l) upon controlling the temperature at 28°C. Adjustment of the aeration level at 1.5vvm for 3days then controlling the dissolved oxygen (DO) at 30% saturation further increased the yield to 17.8g/l. The optimum pH was 6.5 for 3days then 7 thereafter when a production yield of 16.1g/l was recorded using an aeration rate of 1.5vvm. Controlling the pH at constant value (6.5 or 7) all through the fermentation process decreased the yield by 5–21%. Controlling the temperature at 30°C for 3days then 28°C thereafter slightly increased the yield by 5% upon using an aeration rate of 1vvm while it decreased upon using an aeration rate of 1.5vvm. Integration of the most optimum conditions increased the production yield by 22% from 13.81 to 17.8g/l.
ISSN:1110-0931
2090-9101
DOI:10.1016/j.bfopcu.2013.02.002