Group I Metabotropic Glutamate Receptors Modulate Motility and Enteric Neural Activity in the Mouse Colon

Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2023-01, Vol.13 (1), p.139
Main Authors: Leembruggen, Anita J L, Lu, Yuqing, Wang, Haozhe, Uzungil, Volkan, Renoir, Thibault, Hannan, Anthony J, Stamp, Lincon A, Hao, Marlene M, Bornstein, Joel C
Format: Article
Language:English
Subjects:
Animals
Calcium - pharmacology
Calcium imaging
Central nervous system
Central Nervous System - metabolism
Colon
colonic motor complexes
enteric glia
Enteric nervous system
Ethics
Experiments
gastrointestinal tract
Glutamic Acid - metabolism
Glutamic acid receptors (metabotropic)
Hybridization
Laboratories
metabotropic glutamate receptors
Mice
Motility
Myenteric plexus
Nervous system
Neurons
Neurons - metabolism
Neurotransmission
Receptor mechanisms
Software
Synaptic plasticity
Synaptic Transmission
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Summary:Glutamate is the major excitatory neurotransmitter in the central nervous system, and there is evidence that Group-I metabotropic glutamate receptors (mGlu1 and mGlu5) have established roles in excitatory neurotransmission and synaptic plasticity. While glutamate is abundantly present in the gut, it plays a smaller role in neurotransmission in the enteric nervous system. In this study, we examined the roles of Group-I mGlu receptors in gastrointestinal function. We investigated the expression of (mGlu1) and (mGlu5) in the mouse myenteric plexus using RNAscope in situ hybridization. Live calcium imaging and motility analysis were performed on ex vivo preparations of the mouse colon. mGlu5 was found to play a role in excitatory enteric neurotransmission, as electrically-evoked calcium transients were sensitive to the mGlu5 antagonist MPEP. However, inhibition of mGlu5 activity did not affect colonic motor complexes (CMCs). Instead, inhibition of mGlu1 using BAY 36-7620 reduced CMC frequency but did not affect enteric neurotransmission. These data highlight complex roles for Group-I mGlu receptors in myenteric neuron activity and colonic function.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom13010139