Quantitative Proteomics for the Identification of Differentially Expressed Proteins in the Extracellular Vesicles of Cervical Cancer Cells

The extracellular vesicles (EVs) in a tumoral microenvironment can exert different functions by transferring their content, which has been poorly described in cervical cancer. Here, we tried to clarify the proteomic content of these EVs, comparing those derived from cancerous HPV (+) keratinocytes (...

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Bibliographic Details
Published in:Viruses 2023-03, Vol.15 (3), p.702
Main Authors: Acevedo-Sánchez, Víctor, Martínez-Ruiz, Roy S, Aguilar-Ruíz, Sergio R, Torres-Aguilar, Honorio, Chávez-Olmos, Pedro, Garrido, Efraín, Baltiérrez-Hoyos, Rafael, Romero-Tlalolini, María de Los A
Format: Article
Language:English
Subjects:
Biosynthesis
Biotechnology
cancer
Cell adhesion
Cell culture
Cervical cancer
Chromatography, Liquid
Diagnosis
exosomes
Extracellular vesicles
Extracellular Vesicles - metabolism
Female
Genetic aspects
HeLa Cells
HPV
Human papillomavirus
Humans
Immune response
Keratinocytes
Lipids
Metastases
Microenvironments
Papillomavirus Infections - metabolism
Proteins
Proteins - metabolism
Proteolysis
Proteomics
Signal transduction
Tandem Mass Spectrometry
Transmission electron microscopy
Tumor Microenvironment
Uterine Cervical Neoplasms - metabolism
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Summary:The extracellular vesicles (EVs) in a tumoral microenvironment can exert different functions by transferring their content, which has been poorly described in cervical cancer. Here, we tried to clarify the proteomic content of these EVs, comparing those derived from cancerous HPV (+) keratinocytes (HeLa) versus those derived from normal HPV (-) keratinocytes (HaCaT). We performed a quantitative proteomic analysis, using LC-MS/MS, of the EVs from HeLa and HaCaT cell lines. The up- and downregulated proteins in the EVs from the HeLa cell line were established, along with the cellular component, molecular function, biological processes, and signaling pathways in which they participate. The biological processes with the highest number of upregulated proteins are cell adhesion, proteolysis, lipid metabolic process, and immune system processes. Interestingly, three of the top five signaling pathways with more up- and downregulated proteins are part of the immune response. Due to their content, we can infer that EVs can have a significant role in migration, invasion, metastasis, and the activation or suppression of immune system cells in cancer.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15030702