Identification of the Genes of the Plant Pathogen Pseudomonas syringae MB03 Required for the Nematicidal Activity Against Caenorhabditis elegans Through an Integrated Approach

Nematicidal potential of the common plant pathogen has been recently identified against . The current study was designed to investigate the detailed genetic mechanism of the bacterial pathogenicity by applying comparative genomics, transcriptomics, mutant library screening, and protein expression. R...

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Bibliographic Details
Published in:Frontiers in microbiology 2022-03, Vol.13, p.826962-826962
Main Authors: Ali, Muhammad, Gu, Tong, Yu, Xun, Bashir, Anum, Wang, Zhiyong, Sun, Xiaowen, Ashraf, Naeem Mahmood, Li, Lin
Format: Article
Language:English
Subjects:
Caenorhabditis elegans
gut colonization
Microbiology
pathogenomics
Pseudomonas syringae
transcriptomics
transposon mutant library
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Summary:Nematicidal potential of the common plant pathogen has been recently identified against . The current study was designed to investigate the detailed genetic mechanism of the bacterial pathogenicity by applying comparative genomics, transcriptomics, mutant library screening, and protein expression. Results showed that strain MB03 could kill in the liquid assay by gut colonization. The genome of MB03 was sequenced and comparative analysis including multi locus sequence typing, and genome-to-genome distance placed MB03 in phylogroup II of . Furthermore, comparative genomics of MB03 with nematicidal strains of (PAO1 and PA14) predicted 115 potential virulence factors in MB03. However, genes for previously reported nematicidal metabolites, such as phenazine, pyochelin, and pyrrolnitrin, were found absent in the MB03 genome. Transcriptomics analysis showed that the growth phase of the pathogen considerably affected the expression of virulence factors, as genes for the flagellum, glutamate ABC transporter, / , / , type VI secretion system, and serralysin were highly up-regulated when stationary phase MB03 cells interacted with Additionally, screening of a transposon insertion mutant library led to the identification of other nematicidal genes such as , and . Finally, the nematicidal activity of selected proteins was confirmed by heterologous expression in .
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.826962