Long-lasting effects of lipopolysaccharide on the reproduction and splenic transcriptome of hens and their offspring

• Published in: Ecotoxicology and environmental safety 2022-06, Vol.237, p.113527-113527, Article 113527
• Main Authors: Liu, Lei, Wang, Di, Li, Xingzheng, Adetula, Adeyinka Abiola, Khan, Adnan, Zhang, Bing, Liu, Huagui, Yu, Ying, Chu, Qin
• Format: Article
• Language: English
• Subjects: Animal Feed; Animals; Chickens; Egg-laying rate; Female; Immunity; Laying hens; Lipopolysaccharides - metabolism; Long-lasting effect; LPS maternal stimulation; Reproduction; Spleen; Transcriptome
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.113527

Lipopolysaccharide (LPS) is ubiquitous in the environment and is released after the death of gram-negative bacteria, which may be related to inflammation and immunosuppression. However, its impact on the reproduction of animals and their offspring, especially the underlying mechanism need further elucidation. Here, we used laying hens as a model organism to investigate the effects of maternal exposure to LPS (LPS maternal stimulation) on animal and their offspring’s immunity and reproductive performance, as well as the regulatory role of the transcriptome. We found that the LPS maternal stimulation could reduce the egg-laying rate of hens and their offspring, especially during the early and late laying stages. The transcriptome study of the spleen in F0, F1 and F2 generations showed that the maternal stimulation of the LPS affects the patterns of gene expression in laying hens, and this change has a long-lasting effect. Further analysis of DEGs and their enrichment pathways found that the LPS maternal stimulation mainly affects the reproduction and immunity of laying hens and their offspring. The DEGs such as AVD, HPS5, CATHL2, S100A12, EXFABP, RSFR, LY86, PKD4, XCL1, FOS, TREM2 and MST1 may play an essential role in the regulation of the immunity and egg-laying rate of hens. Furthermore, the MMR1L3, C3, F13A1, LY86 and GDPD2 genes with heritable effects are highly correlated with the egg-laying rate, may have an important reference value for further research. Our study reveals the profound implications of LPS exposure on immunity and reproduction of offspring, elaborating the impact of immune alteration on the egg-laying rate, emphasizing the regulatory role of intergenerational transmission of the transcriptome, implying that the environment parents being exposed to has an important impact on offspring. [Display omitted] •LPS stimulation induced a drop in reproduction of laying hens and their offspring.•LPS caused long-lasting effects on splenic transcriptome of unchallenged offspring.•LPS-caused differentially expressed genes are related to immunity and reproduction.•The environment parents being exposed to has an important impact on offspring.