Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology

Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular metabolism, the optimization of metabolic pathways for maximal...

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Bibliographic Details
Published in:Synthetic and systems biotechnology 2018-06, Vol.3 (2), p.90-96
Main Authors: Jiang, Lihong, Zhao, Jiarun, Lian, Jiazhang, Xu, Zhinan
Format: Article
Language:English
Subjects:
Cell-free protein synthesis
Genetic circuits
Metabolic engineering
Metabolic pathway optimization
Synthetic biology
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Summary:Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular metabolism, the optimization of metabolic pathways for maximal production represents a grand challenge and an unavoidable barrier for metabolic engineering. Recently, cell-free protein synthesis system (CFPS) has been emerging as an enabling alternative to address challenges in biomanufacturing. This review summarizes the recent progresses of CFPS in rapid prototyping of biosynthetic pathways and genetic circuits (biosensors) to speed up design-build-test (DBT) cycles of metabolic engineering and synthetic biology.
ISSN:2405-805X
2405-805X
DOI:10.1016/j.synbio.2018.02.003