Impact of acute exercise on peripheral blood mononuclear cells nutrient sensing and mitochondrial oxidative capacity in healthy young adults

Regular exercise is associated with changes in peripheral blood mononuclear cell (PBMC) proportions that have enhanced effector functions in young and old adults; however, the effects of acute exercise on PBMC nutrient sensors and metabolic function in active young adults is unknown. To fill this ga...

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Bibliographic Details
Published in:Physiological reports 2021-12, Vol.9 (23), p.e15147-n/a
Main Authors: Theall, Bailey, Stampley, James, Cho, Eunhan, Granger, Joshua, Johannsen, Neil M., Irving, Brian A., Spielmann, Guillaume
Format: Article
Language:English
Subjects:
acute exercise
Adult
Antibodies
Body mass index
CD4 antigen
CD69 antigen
CD8 antigen
Cell Respiration - physiology
Cloning
Cytomegalovirus
Electron transport
Energy Metabolism - physiology
Exercise
Exercise - physiology
Female
Flow cytometry
Humans
Leukocytes (mononuclear)
Leukocytes, Mononuclear - metabolism
lymphocyte activation
Lymphocyte Activation - physiology
Lymphocytes
Male
Metabolism
Mitochondria
Mitochondria - metabolism
Original
Oxidative Phosphorylation
Oxidative Stress - physiology
Oxygen Consumption - physiology
PBMC
Peripheral blood mononuclear cells
Physical fitness
Physiology
Respiration
Sensors
Workloads
Young Adult
Young adults
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Summary:Regular exercise is associated with changes in peripheral blood mononuclear cell (PBMC) proportions that have enhanced effector functions in young and old adults; however, the effects of acute exercise on PBMC nutrient sensors and metabolic function in active young adults is unknown. To fill this gap, activation status and nutrient‐sensing mechanisms of PBMCs isolated from 21 healthy active adults (20–35 yr; 36.5 ± 6.3 V̇O2peak) were characterized before and after 30 min of moderate‐to‐vigorous cycling (65%–75% V̇O2peak). In addition, changes in PBMC mitochondrial respiratory function in response to exercise were assessed using high‐resolution respirometry. There was an increase in the number of activated CD69+/CD4 (79% increase) and CD69+/CD8 (166% increase) T‐cells in response to the acute bout of exercise, while the nutrient‐sensing mechanisms remained unchanged. PBMC mitochondrial respiration did not increase on a cell‐per‐cell basis, however, mitochondrial oxidative capacity (OXPHOS) increased at the tissue level (18.6 pmol/(s*ml blood) versus 29.3 pmol/(s*ml blood); p 
ISSN:2051-817X
DOI:10.14814/phy2.15147