Myeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling

• Published in: Scientific reports 2022-10, Vol.12 (1), p.16656-16656, Article 16656
• Main Authors: Tomimatsu, Masashi, Matsumoto, Kotaro, Ashizuka, Moe, Kumagai, Shohei, Tanaka, Shota, Nakae, Takafumi, Yokota, Kosei, Kominami, Shunsuke, Kajiura, Ryota, Okuzaki, Daisuke, Motooka, Daisuke, Shiraishi, Aki, Abe, Takaya, Matsuda, Hideo, Okada, Yoshiaki, Maeda, Makiko, Seno, Shigeto, Obana, Masanori, Fujio, Yasushi
• Format: Article
• Language: English
• Subjects: 631/80; 692/4019; Ablation; Angiogenesis; Antibodies; Calcification (ectopic); Cardiac function; Cbfa-1 protein; Cell culture; Collagen; Culture media; Cytokines; Endothelial cells; Fibrosis; Gene expression; Genetic engineering; Heart attacks; Homeostasis; Humanities and Social Sciences; Inflammation; Macrophages; multidisciplinary; Myeloid cells; Myocardial infarction; Myocardium; Neutrophils; Nucleotide sequence; Osteoblastogenesis; Pharmaceutical sciences; Proteins; Science; Science (multidisciplinary); Tibia; Transcription factors; Ventricle
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-21202-7

Runt-related transcription factor 2 (Runx2), a regulator of osteoblast differentiation, is pathologically involved in vascular calcification; however, the significance of Runx2 in cardiac homeostasis remains unclear. Here, we investigated the roles of Runx2 in cardiac remodeling after myocardial infarction (MI). The expression of Runx2 mRNA and protein was upregulated in murine hearts after MI. Runx2 was expressed in heart-infiltrating myeloid cells, especially in macrophages, at the border zone of post-infarct myocardium. To analyze the biological functions of Runx2 in cardiac remodeling, myeloid cell-specific Runx2 deficient (CKO) mice were exposed to MI. After MI, ventricular weight/tibia length ratio was increased in CKO mice, concomitant with severe cardiac dysfunction. Cardiac fibrosis was exacerbated in CKO mice, consistent with the upregulation of collagen 1a1 expression. Mechanistically, immunohistochemical analysis using anti-CD31 antibody showed that capillary density was decreased in CKO mice. Additionally, conditioned culture media of myeloid cells from Runx2 deficient mice exposed to MI induced the tube formation of vascular endothelial cells to a lesser extent than those from control mice. RNA-sequence showed that the expression of pro-angiogenic or anti-angiogenic factors was altered in macrophages from Runx2-deficient mice. Collectively, Runx2 + myeloid cells infiltrate into post-infarct myocardium and prevent adverse cardiac remodeling, at least partially, by regulating endothelial cell function.