Short link N acts as a disease modifying osteoarthritis drug

• Published in: European cells & materials 2019-05, Vol.37, p.347-359
• Main Authors: Antoniou, J, Epure, L M, Grant, M P, Richard, H, Sampalis, J, Roughley, P J, Laverty, S, Mwale, F
• Format: Article
• Language: English
• Subjects: Animals; Anterior Cruciate Ligament - drug effects; Anterior Cruciate Ligament - metabolism; Anterior Cruciate Ligament Injuries - drug therapy; Anterior Cruciate Ligament Injuries - metabolism; bioactive peptides; cartilage repair; Cartilage, Articular - drug effects; Cartilage, Articular - metabolism; Disease Models, Animal; Disease Progression; Extracellular Matrix Proteins - metabolism; Extracellular Matrix Proteins - pharmacology; Femur - drug effects; Femur - metabolism; Injections, Intra-Articular - methods; Knee Joint - drug effects; Knee Joint - metabolism; Osteoarthritis; Osteoarthritis - drug therapy; Osteoarthritis - metabolism; Proteoglycans - metabolism; Proteoglycans - pharmacology; Rabbits; short link N; Tibia - drug effects; Tibia - metabolism; tissue engineering
• ISSN: 1473-2262
• DOI: 10.22203/eCM.v037a21

Osteoarthritis (OA) is a degenerative joint disease characterised by a progressive degradation of articular cartilage and underlaying bone and is associated with pain and disability. Currently, there is no medical treatment to reverse or even retard OA. Based on our previous reports, where we establish the repair potential of short Link N (sLN) in the intervertebral disc, a cartilage-like tissue, we hypothesise that sLN may hold similar promises in the repair of articular cartilage. This study aimed to determine if sLN, could prevent OA disease progression. Skeletally mature New Zealand white rabbits underwent unilateral anterior cruciate ligament transection (ACLT) of their left femorotibial joints to induce joint degeneration typical of OA. Beginning 3 weeks post-operatively, and every three weeks thereafter for 12 weeks, either saline (1 mL) or sLN (100 μg in 1 mL saline) was injected intraarticularly into the operated knee. Six additional rabbits underwent sham surgery but without ACLT or post-operative injections. The effects on gross joint morphology and cartilage histologic changes were evaluated. In the Saline group, prominent erosion of articular cartilage occurred in both femoral condyle compartments and the lateral compartment of the tibial plateau while, sLN treatment reduced the severity of the cartilage damage in these compartments of the knee showing erosion. Furthermore, statistically significant differences were detected between the joint OA score of the saline and sLN treated groups (p = 0.0118). Therefore, periodic intraarticular injection of sLN is a promising nonsurgical treatment for preventing or retarding OA progression, by reducing cartilage degradation.