LncRNA NCK1-AS1 in plasma distinguishes oral ulcer from early-stage oral squamous cell carcinoma

Oral squamous cell carcinoma (OSCC) at early stages can be misdiagnosed as an oral ulcer (OU) due to similar symptoms, such as chronic and indurated ulcer. LncRNA NCK1-AS1 has been characterized as a key player in cervical cancer, while its role in OSCC is unknown. All participants were selected at...

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Bibliographic Details
Published in:Journal of biological research (Thessalonikē, Greece) Greece), 2020-11, Vol.27 (1), p.1-7, Article 16
Main Authors: Le, Fei, Ou, Yangqian, Luo, Ping, Zhong, Xiaoming
Format: Article
Language:English
Subjects:
Age
Analysis
Cancer
Cell adhesion & migration
Cell migration
Cervical cancer
Cervix
Confidence intervals
Deoxyribonucleic acid
Diagnostic systems
DNA
DNA methylation
Gene expression
Infection
lncRNA NCK1-AS1
Metastases
MicroRNAs
miR-100
Neoplasms
Oral cancer
Oral squamous cell carcinoma
Oral ulcer
Plasma
Squamous cell carcinoma
Symptoms
Transfection
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Summary:Oral squamous cell carcinoma (OSCC) at early stages can be misdiagnosed as an oral ulcer (OU) due to similar symptoms, such as chronic and indurated ulcer. LncRNA NCK1-AS1 has been characterized as a key player in cervical cancer, while its role in OSCC is unknown. All participants were selected at Jiangxi Province Tumor Hospital from December 2016 to December 2018. Expression levels of NCK1-AS1 and miR-100 in plasma from both OSCC and OU patients were measured by RT-qPCR. Diagnostic analysis was performed through ROC curve. Potential interactions between NCK1-AS1 and miR-100 were detected by cell transfection experiments. Cell invasion and migration were assessed by Transwell assays. The expression of NCK1-AS1 was upregulated in early-stage OSCC patients but not in OU patients. Upregulation of NCK1-AS1 distinguished OSCC patients from OU patients. The expression of miR-100 was inversely correlated with the expression of NCK1-AS1. Overexpression of NCK1-AS1 was followed by promoted OSCC cell invasion and migration. Overexpression of miR-100 did not affect the expression of NCK1-AS1 but inhibited the role of NCK1-AS1. Therefore, NCK1-AS1 may promote the metastasis of OSCC by downregulating miR-100.
ISSN:2241-5793
1790-045X
2241-5793
DOI:10.1186/s40709-020-00126-1