The occurrence of Phellinus torulosus in Apulia and Basilicata (Southern Italy): identification of isolates by morphologic, microscopic and molecular means [Quercus]

Basidiomes of Phellinus torulosus were found in 9 oak woods in Apulia and Basilicata (Southern Italy); from these basidiomes 138 isolates of P. torulosus were obtained and identified by their morphologic, microscopic and molecular characteristics. Based on the type of aerial mycelium (fluffy, cotton...

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Bibliographic Details
Published in:Phytopathologia mediterranea 2004-08, Vol.43 (2), p.289-298
Main Authors: Campanile, G. (Bari Univ. (Italy). Dipartimento di Biologia e Patologia Vegetale), Luisi, N. (Bari Univ. (Italy). Dipartimento di Biologia e Patologia Vegetale)
Format: Article
Language:English
Subjects:
ADN
apulia
basilicata
BASILICATE
BOSQUES
CARIE DU BOIS
dna
ESPOROFOROS
foreste
forests
FORET
IDENTIFICACION
identification
identificazione
marciume del legno
micelio
microscopia
MICROSCOPIE
microscopy
mycelium
nucleotide sequence
pcr
phellinus
POUILLES
PUDRICION DE LA MADERA
puglia
quercus
RESEARCH PAPERS
rflp
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
sequenza nucleotidica
sporofori
SPOROPHORE
sporophores
wood decay
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Summary:Basidiomes of Phellinus torulosus were found in 9 oak woods in Apulia and Basilicata (Southern Italy); from these basidiomes 138 isolates of P. torulosus were obtained and identified by their morphologic, microscopic and molecular characteristics. Based on the type of aerial mycelium (fluffy, cottony or powdery) and its growth, 9 morphotypes were identified. The morphology of the cultures was not correlated with the microscopic characters of the 9 morphotypes. Molecular analysis, such as intergenic transcribed spacers-restriction fragment length polymorphism (ITS-RFLP) and sequencing of the ITS region, confirmed the results obtained with microscopy analysis [Sono stati rinvenuti basidiomi di Phellinus torulosus in 9 querceti in Puglia e Basilicata (Italia Meridionale); da questi basidiomi sono stati ottenuti 138 isolati di P. torulosus, in base alle caratteristiche morfologiche, microscopiche e molecolari. In base al tipo di micelio aereo (lanuginoso, cotonoso o polverulento) e alla sua crescita, sono stati identificati 9 morfotipi. La morfologia delle colture non è risultata correlata con i caratteri microscopici dei 9 morfotipi. L´analisi molecolare, come ITS-RFLP e sequenziamento della regione ITS, ha confermato i risultati dell´analisi microscopica.]
ISSN:0031-9465
1593-2095
DOI:10.14601/Phytopathol_Mediterr-1757