Biological effects of intraoperative radiation therapy: histopathological changes and immunomodulation in breast cancer patients

Intraoperative radiation therapy (IORT) delivers a single accelerated radiation dose to the breast tumor bed during breast-conserving surgery (BCS). The synergistic biologic effects of simultaneous surgery and radiation remain unclear. This study explores the cellular and molecular changes induced b...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in immunology 2024-04, Vol.15, p.1373497
Main Authors: Orozco, Javier I J, Valdez, Betsy J, Matsuba, Chikako, Simanonok, Michael P, Ensenyat-Mendez, Miquel, Ramiscal, Judi Anne B, Salomon, Matthew P, Takasumi, Yuki, Grumley, Janie G
Format: Article
Language:English
Subjects:
Adult
Aged
breast neoplasms
Breast Neoplasms - immunology
Breast Neoplasms - pathology
Breast Neoplasms - radiotherapy
Combined Modality Therapy
Female
Humans
immune response
Immunology
Immunomodulation - radiation effects
Intraoperative Care
intraoperative radiation therapy-IORT
Mastectomy, Segmental
Middle Aged
squamous metaplasia
tumor microenvironment
Tumor Microenvironment - immunology
Tumor Microenvironment - radiation effects
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Intraoperative radiation therapy (IORT) delivers a single accelerated radiation dose to the breast tumor bed during breast-conserving surgery (BCS). The synergistic biologic effects of simultaneous surgery and radiation remain unclear. This study explores the cellular and molecular changes induced by IORT in the tumor microenvironment and its impact on the immune response modulation. Patients with hormone receptor (HR)-positive/HER2-negative, ductal carcinoma (DCIS), or early-stage invasive breast carcinoma undergoing BCS with margin re-excision were included. Histopathological evaluation and RNA-sequencing in the re-excision tissue were compared between patients with IORT (n=11) vs. non-IORT (n=11). Squamous metaplasia with atypia was exclusively identified in IORT specimens (63.6%, =0.004), mimicking DCIS. We then identified 1,662 differentially expressed genes (875 upregulated and 787 downregulated) between IORT and non-IORT samples. Gene ontology analyses showed that IORT was associated with the enrichment of several immune response pathways, such as inflammatory response, granulocyte activation, and T-cell activation (
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2024.1373497