Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)

Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays. In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The conserved region with...

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Bibliographic Details
Published in:BMC veterinary research 2020-07, Vol.16 (1), p.249-249, Article 249
Main Authors: Yuan, Lin, Liu, Yingyi, Chen, Yana, Gu, Xiaoxue, Dong, Hao, Zhang, Shuo, Han, Tao, Zhou, Zhi, Song, Xiaohui, Wang, Chuanbin
Format: Article
Language:English
Subjects:
Animal diseases
Animals
Capsid Proteins - genetics
Circoviridae Infections - diagnosis
Circoviridae Infections - veterinary
Circoviridae Infections - virology
Circovirus - genetics
Circovirus - isolation & purification
Conserved sequence
Deoxyribonucleic acid
Dermatitis
Disease control
DNA
DNA Primers
DNA probes
Fluorescence
Genomes
Hogs
Methodology
Methods
Nucleotide sequence
Polymerase chain reaction
Porcine circovirus type 3
Real-time PCR
Real-Time Polymerase Chain Reaction - methods
Real-Time Polymerase Chain Reaction - veterinary
Sensitivity and Specificity
Sus scrofa
Swine
Swine Diseases - diagnosis
Swine Diseases - virology
Virus
Viruses
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Summary:Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays. In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The conserved region within Capsid genome of PCV3 was selected for the design of primer pairs and probes. After optimizing, a primer pair and probe was screened, providing high sensitivity (10 copies/μL) and specificity (no cross reaction with other porcine viruses or common bacterium). In addition, this method was applied in the detection of 110 clinical samples, and the performance was compared with other previously reported PCR and real-time PCR methods. This method provided higher detection rate. A real-time fluorescence PCR assay has been developed for the detection of PCV3, with high sensitivity and specificity, exhibiting good performance in detecting clinical samples.
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-020-02435-y