Bovine herpes virus-1 (BoHV-1) detection in dairy cattle with reproductive problems in Sudan

The present work aimed to observe the infection pattern of Bovine herpes virus-1 (BoHV-1) in dairy cattle with reproductive problems in Sudan. A total of 140 samples comprising of vaginal swab (n=97), placenta (n=15), whole blood (n=19), uterine fluid (n=1), and serum (n=8) were collected from 16 da...

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Bibliographic Details
Published in:Journal of advanced veterinary and animal research 2015-06, Vol.2 (2), p.185-189
Main Authors: Elhassan, Amira, Fadol, Mohamed, Salih, Mohamed, Hussein, Abdel
Format: Article
Language:English
Subjects:
Abortion
Alcohols
Antigens
BoHV-1
Cattle
Cell culture
Chloroform
Dairy cattle
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Enzymes
Farms
Herpes viruses
Immunoglobulins
Infections
Infertility
Methods
Phenols
Placenta
Polymerase chain reaction
Reproductive problems
Research centers
Sudan
Uterus
Vagina
Viruses
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Summary:The present work aimed to observe the infection pattern of Bovine herpes virus-1 (BoHV-1) in dairy cattle with reproductive problems in Sudan. A total of 140 samples comprising of vaginal swab (n=97), placenta (n=15), whole blood (n=19), uterine fluid (n=1), and serum (n=8) were collected from 16 dairy herds showing particularly high rate of abortion and infertility in Khartoum State. The samples were used for virus isolation, and were tested by EnzymeLinked Immunosorbent Assay (ELISA) and polymerase chain reaction (PCR). No virus could be isolated from the samples inoculated for isolation in cell culture. Out of 80 specimens tested by ELISA, 7 (8.75%) were found to be positive, and one sample was doubtful. Using PCR, 11 (10.7%) out of 103 samples were found to be positive. When comparing between two methods for DNA extraction, the DNA extracted by commercial kit was found to be better in quality as compared to the DNA extracted using phenol/chloroform/isoamyl-alcohol method. The study confirmed the presence of BoHV-1 in cattle farms with reproductive problems in Sudan.
ISSN:2311-7710
2311-7710
DOI:10.5455/javar.2015.b81