Phytochemical and Biological Screening of Leaf, Bark and Fruit Extracts from Ilex dipyrena Wall

• Published in: Life (Basel, Switzerland) Switzerland), 2021-08, Vol.11 (8), p.837
• Main Authors: Ali, Amjad, Khalil, Atif Ali Khan, Khuda, Fazli, Nazir, Nausheen, Ullah, Riaz, Bari, Ahmed, Haider, Adnan, Jamal, Syed Babar, Ahmad, Sareer, Khan, Zahid, Shah, Syed Muhammad Mukarram, Shah, Syed Wadood Ali, Nasir, Abdul, Ali, Abid, Zahoor, Muhammad, Jan, Samin
• Format: Article
• Language: English
• Subjects: Acetic acid; Amphetamines; Analgesia; analgesic; Analgesics; Animals; antioxidant; Antioxidants; Bark; Bioactive compounds; Caustic soda; Drug dosages; Fever; Flavonoids; Formaldehyde; Free radicals; Fruits; GC-MS analysis; Ilex dipyrena wall; Leaves; Medicinal plants; Natural products; Nonsteroidal anti-inflammatory drugs; Pain; Pain perception; Palmitic acid; Phenylephrine; Phytochemicals; Sodium
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life11080837

The Aquifoliaceae is an important family and has been used traditionally for some time. One of the members of this family is the Ilex dipyrena wall, which itself possesses a potential medicinal importance. This plant is traditionally used for the treatment of various ailments including pain, swelling, burns, and fever. The current study was designed to screen out the antioxidant and analgesic potential of this plant and to verify its traditional uses, along with its phytochemical profile. Extracts were subjected to antioxidant, analgesic, and phytochemical analysis using DPPH, chemical-induced (acetic acid and formalin) nociception models and GC-MS analysis, respectively. The leaf, bark, and fruit extracts showed significant antioxidant activity compared to that of standard. Likewise, all the extracts demonstrated significant (p < 0.01) analgesic activity in a mice model. In acetic acid induced analgesia, the leaf, bark, and fruit extracts caused 51.64, 56.13 and 59.52% inhibition, respectively at a dose of 100 mg/kg while at 200 mg/kg it showed 83.01, 71.69 and 75.47% inhibition, respectively. In Formalin-induced paw-licking assay, fruit extract showed 59.42 and 64.19% inhibition at 200 mg/kg dose in the first and second phase, respectively. The GC-MS analysis revealed the presence of cathinone, phenylpropanolamine, dl-phenylephrine, amphetamine, myristic acid, and palmitic acid. Results of the study suggest that crude extracts from different parts of this plant may be a useful source for the development of novel analgesics. However, further investigation in terms of isolation of bioactive compounds and their toxicological evaluations are needed to validate the observed results.